| RRC ID |
67857
|
| 著者 |
Sun Y, Ito S, Nishio N, Tanaka Y, Chen N, Isobe K.
|
| タイトル |
Acrolein induced both pulmonary inflammation and the death of lung epithelial cells.
|
| ジャーナル |
Toxicol Lett
|
| Abstract |
Acrolein, a compound found in cigarette smoke, is a major risk factor for respiratory diseases. Previous research determined that both acrolein and cigarette smoke produced reactive oxygen species (ROS). As many types of pulmonary injuries are associated with inflammation, this study sought to ascertain the extent to which exposure to acrolein advanced inflammatory state in the lungs. Our results showed that intranasal exposure of mice to acrolein increased CD11c(+)F4/80(high) macrophages in the lungs and increased ROS formation via induction of NF-κB signaling. Treatment with acrolein activated macrophages and led to their increased production of ROS and expression of several key pro-inflammatory cytokines. In in vitro studies, acrolein treatment of bone marrow-derived GM-CSF-dependent immature macrophages (GM-IMs), activated the cells and led to their increased production of ROS and expression of several key pro-inflammatory cytokines. Acrolein treatment of macrophages induced apoptosis of lung epithelial cells. Inclusion of an inhibitor of ROS formation markedly decreased acrolein-mediated macrophage activation and reduced the extent of epithelial cell death. These results indicate that acrolein can cause lung damage, in great part by mediating the increased release of pro-inflammatory cytokines/factors by macrophages.
|
| 巻・号 |
229(2)
|
| ページ |
384-92
|
| 公開日 |
2014-9-2
|
| DOI |
10.1016/j.toxlet.2014.06.021
|
| PII |
S0378-4274(14)00266-5
|
| PMID |
24999835
|
| MeSH |
Acrolein / toxicity*
Animals
Apoptosis / drug effects*
Blotting, Western
Cytokines / analysis
Cytokines / biosynthesis
Female
Flow Cytometry
Lung / chemistry
Lung / cytology
Lung / drug effects*
Macrophage Activation / drug effects
Mice
Mice, Inbred C57BL
NF-kappa B / drug effects
Pneumonia / chemically induced*
Reactive Oxygen Species / analysis
Real-Time Polymerase Chain Reaction
Respiratory Mucosa / drug effects*
Signal Transduction / drug effects
Transcription Factor RelA / drug effects
|
| IF |
3.569
|
| リソース情報 |
| ヒト・動物細胞 |
LLC(RCB0558) |
