RRC ID 67935
Author Uto T, Suangkaew N, Morinaga O, Kariyazono H, Oiso S, Shoyama Y.
Title Eriobotryae folium extract suppresses LPS-induced iNOS and COX-2 expression by inhibition of NF-kappaB and MAPK activation in murine macrophages.
Journal Am J Chin Med
Abstract Eriobotryae folium (EF), the dried leaves of Eriobotrya japonica (Thunb.) Lindl. has been traditionally used to treat various diseases such as chronic bronchitis, cough, inflammation, skin diseases, and diabetes. In this study, we examined the effects of Eriobotryae folium extract (EFE) on lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2(PGE2) in RAW264 murine macrophage cells. EFE suppressed LPS-induced NO and PGE2 production in a dose-dependent manner. Consistent with these observations, EFE reduced the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both protein and mRNA levels. Furthermore, EFE significantly inhibited LPS-induced NF-kappaB binding activity, which was associated with the inhibition of IkappaB-alpha degradation. EFE also attenuated LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun N-terminal kinase (JNK). These results suggest that the anti-inflammatory properties of EF might result from inhibition of iNOS and COX-2 expression through the downregulation of NF-kappaB activation and MAPK phosphorylation in LPS-stimulated RAW264 cells.
Volume 38(5)
Pages 985-94
Published 2010-1-1
DOI 10.1142/S0192415X10008408
PII S0192415X10008408
PMID 20821828
MeSH Animals Anti-Inflammatory Agents / pharmacology* Cyclooxygenase 2 / genetics Cyclooxygenase 2 / metabolism* Cyclooxygenase 2 Inhibitors / pharmacology Dinoprostone / biosynthesis* Dose-Response Relationship, Drug Down-Regulation Drugs, Chinese Herbal / pharmacology* Eriobotrya* I-kappa B Proteins / metabolism Lipopolysaccharides Macrophages / drug effects* Macrophages / metabolism Mice Mitogen-Activated Protein Kinases / antagonists & inhibitors NF-KappaB Inhibitor alpha NF-kappa B / antagonists & inhibitors Nitric Oxide / biosynthesis Nitric Oxide Synthase Type II / biosynthesis* Nitric Oxide Synthase Type II / genetics Phosphorylation Plant Leaves RNA, Messenger / metabolism
IF 3.682
Human and Animal Cells RAW 264(RCB0535)