論文 - 詳細
| RRC ID | 67977 |
|---|---|
| 著者 | Aramaki Y, Takano S, Tsuchiya S. |
| タイトル | Cationic liposomes induce macrophage apoptosis through mitochondrial pathway. |
| ジャーナル | Arch Biochem Biophys |
| Abstract |
To clarify the mechanism of apoptosis of the macrophage-like cell line RAW264.7 induced by cationic liposomes, we focused on the mitochondria and investigated the changes in mitochondrial membrane potential and the release of cytochrome c following treatment of cationic liposomes composed of stearylamine (SA-liposomes). SA-liposomes induced mitochondrial membrane depolarization and also the release of cytochrome c from mitochondria. Caspase-3 was also activated by SA-liposome treatment. Pretreatment of cells with N-acetylcysteine, a scavenger of reactive oxygen species (ROS), conferred resistance to the induction of the membrane depolarization, cytochrome c release, and caspase-3 activation by SA-liposomes. These results indicated that SA-liposomes caused the apoptosis in RAW264.7 cells through the mitochondrial pathway, and ROS generation was required for this phenomenon. |
| 巻・号 | 392(2) |
| ページ | 245-50 |
| 公開日 | 2001-8-15 |
| DOI | 10.1006/abbi.2001.2458 |
| PII | S0003-9861(01)92458-0 |
| PMID | 11488598 |
| MeSH | Acetylcysteine / pharmacology Animals Annexin A5 / pharmacology Apoptosis* Blotting, Western Caspase 3 Caspases / metabolism Cations* Cell Line Cytochrome c Group / metabolism DNA Fragmentation Enzyme Activation Flow Cytometry Free Radical Scavengers / pharmacology Liposomes / metabolism* Macrophages / pathology* Membrane Potentials Mice Mitochondria / metabolism* |
| IF | 3.391 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |