Reference - Detail
| RRC ID | 67978 |
|---|---|
| Author | Takano S, Aramaki Y, Tsuchiya S. |
| Title | Lipoxygenase may be involved in cationic liposome-induced macrophage apoptosis. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The purpose of this study was to determine the source of reactive oxygen species (ROS) generation and the contribution of ROS to the apoptosis of RAW264.7 cells induced by cationic liposomes. Cationic liposome-induced apoptosis was inhibited by lipoxygenase inhibitors, but not inhibitors of NADPH-oxidase, xanthine oxidase or cyclooxygenase. ROS generation induced by cationic liposomes was also inhibited by the lipoxygenase inhibitor NDGA. Furthermore, lipid peroxidation was observed following liposome treatment, but the apoptosis was not inhibited by the antioxidant alpha-tocopherol. These findings suggested that lipoxygenase is responsible for ROS generation, and ROS but not lipid peroxidation acts as a key mediator in the progress of apoptosis induced by cationic liposomes. |
| Volume | 288(1) |
| Pages | 116-20 |
| Published | 2001-10-19 |
| DOI | 10.1006/bbrc.2001.5731 |
| PII | S0006-291X(01)95731-6 |
| PMID | 11594761 |
| MeSH | Animals Antioxidants / pharmacology Apoptosis* / drug effects Arachidonic Acid / pharmacology Cations Cell Line Cell Nucleus / ultrastructure DNA Fragmentation Enzyme Inhibitors / pharmacology Lipid Peroxidation Liposomes Lipoxygenase / physiology* Lipoxygenase Inhibitors / pharmacology Macrophages / enzymology* Macrophages / ultrastructure Mice Reactive Oxygen Species / metabolism |
| IF | 2.985 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |