論文 - 詳細
| RRC ID | 68003 |
|---|---|
| 著者 | Sun HB, Yokota H. |
| タイトル | Messenger-RNA expression of matrix metalloproteinases, tissue inhibitors of metalloproteinases, and transcription factors in rheumatic synovial cells under mechanical stimuli. |
| ジャーナル | Bone |
| Abstract |
In an effort to elucidate the role of mechanical stimuli in rheumatoid arthritis, we determined mRNA levels of matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2, and three transcription factors (c-fos, ets-1, and ets-2) under two mechanical shearing conditions as well as simulated unloading. Human synovial cell cultures (MH7A and RA99-01), derived from rheumatoid arthritis patients, were grown for 1 h under mechanical stimuli and the transcript level was assayed by the reverse transcription-polymerase-chain reaction procedure. First, gentle shearing, estimated at approximately 1 dyn/cm(2), induced a consistent decrease in mRNA level of MMP-1, MMP-3, MMP-13, and ets-1 and an increase in the transcript level of TIMP-1, TIMP-2, c-fos, and ets-2. Second, intermediate shearing, estimated at approximately 6 dyn/cm(2), elevated the mRNA level of all MMPs, TIMPs, and the three transcription factors. Third, minimum mRNA level of c-fos, ets-1, and ets-2 was achieved under control conditions at rest, gentle shearing, and simulated unloading, respectively. These in vitro results support a stimulus-dependent transcriptional regulation of MMPs, TIMPs, and transcription factors in cell cultures, suggesting a potential role of shear stress in tissue degradation and prevention in rheumatic joints. |
| 巻・号 | 28(3) |
| ページ | 303-9 |
| 公開日 | 2001-3-1 |
| DOI | 10.1016/s8756-3282(00)00454-3 |
| PII | S8756-3282(00)00454-3 |
| PMID | 11248661 |
| MeSH | Arthritis, Rheumatoid / genetics* Arthritis, Rheumatoid / pathology Base Sequence Cells, Cultured DNA Primers Down-Regulation Gelatinases / metabolism Humans Matrix Metalloproteinases / genetics* Physical Stimulation* RNA, Messenger / genetics* Reverse Transcriptase Polymerase Chain Reaction Synovial Membrane / cytology Synovial Membrane / enzymology Synovial Membrane / metabolism* Tissue Inhibitor of Metalloproteinase-1 / genetics* Tissue Inhibitor of Metalloproteinase-2 / genetics* Transcription Factors / genetics* |
| IF | 4.147 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |