RRC ID 68053
著者 Matsui K, Okamoto K, Hasegawa T, Ohtsuka H, Shimasaki T, Ihara K, Goto Y, Aoki K, Aiba H.
タイトル Identification of ksg1 mutation showing long-lived phenotype in fission yeast.
ジャーナル Genes Cells
Abstract Fission yeast is a good model organism for the study of lifespan. To elucidate the mechanism, we screened for long-lived mutants. We found a nonsense mutation in the ksg1+ gene, which encodes an ortholog of mammalian PDK1 (phosphoinositide-dependent protein kinase). The mutation was in the PH domain of Ksg1 and caused defect in membrane localization and protein stability. Analysis of the ksg1 mutant revealed that the reduced amounts and/or activity of the Ksg1 protein is responsible for the increased lifespan. Ksg1 is essential for growth and known to phosphorylate multiple substrates, but the substrate responsible for the long-lived phenotype of ksg1 mutation is not yet known. Genetic analysis showed that deletion of pck2 suppressed the long-lived phenotype of ksg1 mutant, suggesting that Pck2 might be involved in the lifespan extension caused by ksg1 mutation.
巻・号 26(12)
ページ 967-978
公開日 2021-12-1
DOI 10.1111/gtc.12897
PMID 34534388
MeSH Animals Mutation Phenotype Protein Kinases / genetics Schizosaccharomyces* / genetics Schizosaccharomyces pombe Proteins* / genetics
IF 1.655
リソース情報
酵母 FY24018(JY333)