論文 - 詳細
| RRC ID | 68113 |
|---|---|
| 著者 | Taguchi J, Shibata H, Kabata M, Kato M, Fukuda K, Tanaka A, Ohta S, Ukai T, Mitsunaga K, Yamada Y, Nagaoka SI, Yamazawa S, Ohnishi K, Woltjen K, Ushiku T, Ozawa M, Saitou M, Shinkai Y, Yamamoto T, Yamada Y. |
| タイトル | DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency. |
| ジャーナル | Nat Commun |
| Abstract |
In vivo reprogramming provokes a wide range of cell fate conversion. Here, we discover that in vivo induction of higher levels of OSKM in mouse somatic cells leads to increased expression of primordial germ cell (PGC)-related genes and provokes genome-wide erasure of genomic imprinting, which takes place exclusively in PGCs. Moreover, the in vivo OSKM reprogramming results in development of cancer that resembles human germ cell tumors. Like a subgroup of germ cell tumors, propagated tumor cells can differentiate into trophoblasts. Moreover, these tumor cells give rise to induced pluripotent stem cells (iPSCs) with expanded differentiation potential into trophoblasts. Remarkably, the tumor-derived iPSCs are able to contribute to non-neoplastic somatic cells in adult mice. Mechanistically, DMRT1, which is expressed in PGCs, drives the reprogramming and propagation of the tumor cells in vivo. Furthermore, the DMRT1-related epigenetic landscape is associated with trophoblast competence of the reprogrammed cells and provides a therapeutic target for germ cell tumors. These results reveal an unappreciated route for somatic cell reprogramming and underscore the impact of reprogramming in development of germ cell tumors. |
| 巻・号 | 12(1) |
| ページ | 5041 |
| 公開日 | 2021-8-19 |
| DOI | 10.1038/s41467-021-25249-4 |
| PII | 10.1038/s41467-021-25249-4 |
| PMID | 34413299 |
| PMC | PMC8377058 |
| MeSH | Animals Animals, Genetically Modified Cell Differentiation / physiology Cell Line, Tumor Cells, Cultured Cellular Reprogramming / physiology Epigenesis, Genetic Female Genomic Imprinting Humans Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / pathology* Mice Mice, Inbred ICR Neoplasms / genetics Neoplasms / metabolism Neoplasms / pathology* Neoplasms, Germ Cell and Embryonal / genetics Neoplasms, Germ Cell and Embryonal / metabolism Neoplasms, Germ Cell and Embryonal / pathology* Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) HeLa(RCB0007) |