RRC ID 683
著者 Ogihara Y, Ohsawa T.
タイトル Molecular analysis of the complete set of length mutations found in the plastomes of Triticum-Aegilops species.
ジャーナル Genome
Abstract Precise location and nature of each of 14 length mutations detected among chloroplast DNAs of Triticum-Aegilops species by RFLP analysis were determined at the nucleotide sequence level. Each mutation was compared with at least three non-mutated wild-type plastomes as standards. These 14 length mutations were classified into 4 duplications and 10 deletions. One duplication occurred in the small single-copy region close to the border of the inverted repeat, and the remaining 13 length mutations took place in the large single-copy region. All length mutations occurred in the intergenic regions, suggesting that these length mutations do not affect plastid gene expression. Saltatory replication was the cause of all duplications, whereas intramolecular recombination mediated by short direct repeats played a substantial role in the deletions. Recurrent occurrences of certain deletion events were found in some AT-rich regions, which constituted hot spots for deletion. Out of four hypervariable regions detected among the grass plastomes, two (downstream of rbcL and a tRNA gene accumulated region) were still active after differentiation of Triticum and Aegilops complex.
巻・号 45(5)
ページ 956-62
公開日 2002-10-1
DOI 10.1139/g02-046
PMID 12416629
MeSH Base Sequence Chromosome Mapping DNA Transposable Elements DNA, Chloroplast / genetics* Mutation* Poaceae / genetics* Polymorphism, Restriction Fragment Length Sequence Deletion Triticum / genetics*
IF 2.037
引用数 3
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
コムギ Thirty-six alloplasmic lines? six euplasmic lines?