RRC ID 68431
著者 Sasaki M, Kishimoto M, Itakura Y, Tabata K, Intaruck K, Uemura K, Toba S, Sanaki T, Sato A, Hall WW, Orba Y, Sawa H.
タイトル Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells.
ジャーナル Biochem Biophys Res Commun
Abstract The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2.
巻・号 577
ページ 146-151
公開日 2021-11-5
DOI 10.1016/j.bbrc.2021.09.015
PII S0006-291X(21)01309-7
PMID 34517212
PMC PMC8423671
MeSH A549 Cells Alveolar Epithelial Cells / pathology Alveolar Epithelial Cells / virology* COVID-19 / virology* Cell Culture Techniques / methods* Cells, Cultured Disease Susceptibility Gene Expression Regulation, Neoplastic Humans Peptidyl-Dipeptidase A / genetics Peptidyl-Dipeptidase A / metabolism SARS-CoV-2 / physiology* Serine Endopeptidases / genetics Serine Endopeptidases / metabolism Up-Regulation / genetics
IF 2.985
リソース情報
ヒト・動物細胞 A549(RCB0098)