RRC ID |
68431
|
著者 |
Sasaki M, Kishimoto M, Itakura Y, Tabata K, Intaruck K, Uemura K, Toba S, Sanaki T, Sato A, Hall WW, Orba Y, Sawa H.
|
タイトル |
Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2.
|
巻・号 |
577
|
ページ |
146-151
|
公開日 |
2021-11-5
|
DOI |
10.1016/j.bbrc.2021.09.015
|
PII |
S0006-291X(21)01309-7
|
PMID |
34517212
|
PMC |
PMC8423671
|
MeSH |
A549 Cells
Alveolar Epithelial Cells / pathology
Alveolar Epithelial Cells / virology*
COVID-19 / virology*
Cell Culture Techniques / methods*
Cells, Cultured
Disease Susceptibility
Gene Expression Regulation, Neoplastic
Humans
Peptidyl-Dipeptidase A / genetics
Peptidyl-Dipeptidase A / metabolism
SARS-CoV-2 / physiology*
Serine Endopeptidases / genetics
Serine Endopeptidases / metabolism
Up-Regulation / genetics
|
IF |
2.985
|
リソース情報 |
ヒト・動物細胞 |
A549(RCB0098) |