論文 - 詳細
| RRC ID | 68450 |
|---|---|
| 著者 | Koshimura K, Murakami Y, Sohmiya M, Tanaka J, Kato Y. |
| タイトル | Effects of erythropoietin on neuronal activity. |
| ジャーナル | J Neurochem |
| Abstract |
Recently, erythropoietin (EPO) receptors and synthesis of EPO have been identified in the brain. To clarify the effects of EPO on neuronal cells, we investigated the effects of EPO on Ca2+ uptake, intracellular Ca2+ concentration, membrane potential, cell survival, release and biosynthesis of dopamine, and nitric oxide (NO) production in differentiated PC12 cells, which possess EPO receptors. EPO (10(-12)-10(-10) M) increased 45Ca2+ uptake and intracellular Ca2+ concentration in PC12 cells in a dose-related manner; these increases were inhibited by nicardipine (1 microM) or anti-EPO antibody (1:100 dilution). EPO induced membrane depolarization in PC12 cells. After a 5-day culture without serum and nerve growth factor (NGF), viable cell number decreased to 50% of that of the control cells cultured with serum and NGF. EPO (10(-13)-10(-10) M) increased the number of viable cells cultured without serum and NGF; this increase was blunted by nicardipine or anti-EPO antibody. Incubation with EPO (10(-13)-10(-10) M) stimulated mitogen-activated protein kinase activity in PC12 cells. EPO (10(-13)-10(-10) M) increased dopamine release from PC12 cells and tyrosine hydroxylase activity; these increases were sensitive to nicardipine or anti-EPO antibody. Following a 4-h incubation with EPO (10(-14)-10(-10) M), NO production was increased, which was blunted by nicardipine and anti-EPO antibody. In contrast, maximal NO synthase activity was not changed by EPO. These results suggest that EPO stimulates neuronal function and viability via activation of Ca2+ channels. |
| 巻・号 | 72(6) |
| ページ | 2565-72 |
| 公開日 | 1999-6-1 |
| DOI | 10.1046/j.1471-4159.1999.0722565.x |
| PMID | 10349868 |
| MeSH | Animals Antibodies / pharmacology Biological Transport Calcium / metabolism* Calcium-Calmodulin-Dependent Protein Kinases / metabolism Cell Differentiation / drug effects Cell Membrane / drug effects Cell Membrane / physiology* Cell Survival / drug effects Dopamine / metabolism Erythropoietin / immunology Erythropoietin / pharmacology* Humans Membrane Potentials / drug effects Membrane Potentials / physiology Nerve Growth Factors / pharmacology Neurons / cytology Neurons / drug effects Neurons / physiology* Nicardipine / pharmacology Nitric Oxide / metabolism PC12 Cells Rats Recombinant Proteins Tyrosine 3-Monooxygenase / metabolism |
| IF | 4.066 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |