論文 - 詳細
| RRC ID | 6846 |
|---|---|
| 著者 | Fridolfsson HN, Ly N, Meyerzon M, Starr DA. |
| タイトル | UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. |
| ジャーナル | Dev Biol |
| Abstract |
Nuclei migrate during many events, including fertilization, establishment of polarity, differentiation, and cell division. The Caenorhabditis elegans KASH protein UNC-83 localizes to the outer nuclear membrane where it recruits kinesin-1 to provide the major motor activity required for nuclear migration in embryonic hyp7 cells. Here we show that UNC-83 also recruits two dynein-regulating complexes to the cytoplasmic face of the nucleus that play a regulatory role. One consists of the NudE homolog NUD-2 and the NudF/Lis1/Pac1 homolog LIS-1, and the other includes dynein light chain DLC-1, the BicaudalD homolog BICD-1, and the Egalitarian homologue EGAL-1. Genetic disruption of any member of these two complexes caused nuclear migration defects that were enhanced in some double mutant animals, suggesting that BICD-1 and EGAL-1 function in parallel to NUD-2. Dynein heavy chain mutant animals also had a nuclear migration defect, suggesting these complexes function through dynein. Deletion analysis indicated that independent domains of UNC-83 interact with kinesin and dynein. These data suggest a model where UNC-83 acts as the cargo-specific adaptor between the outer nuclear membrane and the microtubule motors kinesin-1 and dynein. Kinesin-1 functions as the major force generator during nuclear migration, while dynein is involved in regulation of bidirectional transport of the nucleus. |
| 巻・号 | 338(2) |
| ページ | 237-50 |
| 公開日 | 2010-2-15 |
| DOI | 10.1016/j.ydbio.2009.12.004 |
| PII | S0012-1606(09)01393-1 |
| PMID | 20005871 |
| PMC | PMC2826220 |
| MeSH | Animals Biological Transport Caenorhabditis elegans Caenorhabditis elegans Proteins / physiology* Cell Nucleus / physiology* Dyneins / metabolism* Kinesins / metabolism* Membrane Proteins Microtubule-Associated Proteins Multiprotein Complexes Nuclear Envelope / chemistry Nuclear Envelope / metabolism* Nuclear Proteins |
| IF | 2.896 |
| 引用数 | 83 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
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| 最多言及媒体 | Blogs |
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| 線虫 | tm3421 |