RRC ID 6848
Author Harumoto T, Ito M, Shimada Y, Kobayashi TJ, Ueda HR, Lu B, Uemura T.
Title Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity.
Journal Dev. Cell
Abstract How global organ asymmetry and individual cell polarity are connected to each other is a central question in studying planar cell polarity (PCP). In the Drosophila wing, which develops PCP along its proximal-distal (P-D) axis, we previously proposed that the core PCP mediator Frizzled redistributes distally in a microtubule (MT)-dependent manner. Here, we performed organ-wide analysis of MT dynamics by introducing quantitative in vivo imaging. We observed MTs aligning along the P-D axis at the onset of redistribution and a small but significant excess of + ends-distal MTs in the proximal region of the wing. This characteristic alignment and asymmetry of MT growth was controlled by atypical cadherins Dachsous (Ds) and Fat (Ft). Furthermore, the action of Ft was mediated in part by PAR-1. All these data support the idea that the active reorientation of MT growth adjusts cell polarity along the organ axis.
Volume 19(3)
Pages 389-401
Published 2010-9-14
DOI 10.1016/j.devcel.2010.08.004
PII S1534-5807(10)00379-5
PMID 20817616
PMC PMC2951474
MeSH Animals Cadherins / physiology* Cell Adhesion Molecules / physiology* Cell Polarity* Drosophila Proteins / physiology* Drosophila melanogaster / embryology* Drosophila melanogaster / genetics Gene Expression Regulation, Developmental / physiology Glycogen Synthase Kinase 3 Microtubules / metabolism* Morphogenesis Protein-Serine-Threonine Kinases / physiology Signal Transduction Subcellular Fractions Wings, Animal / embryology Wings, Animal / metabolism
IF 9.616
Times Cited 76
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
Drosophila