| RRC ID |
68559
|
| 著者 |
Okumura F, Oki N, Fujiki Y, Ikuta R, Osaki K, Hamada S, Nakatsukasa K, Hisamoto N, Hara T, Kamura T.
|
| タイトル |
ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor that mediates cell-cell interactions and positively regulates myogenesis. The cytoplasmic region of Cdon interacts with other proteins to form a Cdon/JLP/Bnip-2/CDC42 complex that activates p38 mitogen-activated protein kinase (MAPK) and induces myogenesis. However, Cdon complex may include other proteins during myogenesis. In this study, we found that Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) ubiquitin ligase is induced during C2C12 differentiation and is included in the Cdon complex. We knocked-down Zswim8 in C2C12 cells to determine the effect of ZSWIM8 on differentiation. However, we detected neither ZSWIM8-dependent ubiquitination nor the degradation of Bnip2, Cdon, or JLP. In contrast, ZSWIM8 knockdown accelerated C2C12 differentiation. These results suggest that ZSWIM8 is a Cdon complex-included myogenic protein that prevents C2C12 differentiation without affecting the stability of Bnip2, Cdon, and JLP.
|
| 巻・号 |
11(1)
|
| ページ |
20880
|
| 公開日 |
2021-10-22
|
| DOI |
10.1038/s41598-021-00306-6
|
| PII |
10.1038/s41598-021-00306-6
|
| PMID |
34686700
|
| PMC |
PMC8536758
|
| MeSH |
Animals
Cell Adhesion Molecules / metabolism
Cell Differentiation / physiology*
Cell Line
Cell Line, Tumor
HEK293 Cells
Humans
K562 Cells
MAP Kinase Signaling System / physiology
Muscle Development / physiology*
Protein Binding / physiology
Ubiquitin-Protein Ligases / metabolism*
p38 Mitogen-Activated Protein Kinases / metabolism
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
