RRC ID 68559
Author Okumura F, Oki N, Fujiki Y, Ikuta R, Osaki K, Hamada S, Nakatsukasa K, Hisamoto N, Hara T, Kamura T.
Title ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation.
Journal Sci Rep
Abstract Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor that mediates cell-cell interactions and positively regulates myogenesis. The cytoplasmic region of Cdon interacts with other proteins to form a Cdon/JLP/Bnip-2/CDC42 complex that activates p38 mitogen-activated protein kinase (MAPK) and induces myogenesis. However, Cdon complex may include other proteins during myogenesis. In this study, we found that Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) ubiquitin ligase is induced during C2C12 differentiation and is included in the Cdon complex. We knocked-down Zswim8 in C2C12 cells to determine the effect of ZSWIM8 on differentiation. However, we detected neither ZSWIM8-dependent ubiquitination nor the degradation of Bnip2, Cdon, or JLP. In contrast, ZSWIM8 knockdown accelerated C2C12 differentiation. These results suggest that ZSWIM8 is a Cdon complex-included myogenic protein that prevents C2C12 differentiation without affecting the stability of Bnip2, Cdon, and JLP.
Volume 11(1)
Pages 20880
Published 2021-10-22
DOI 10.1038/s41598-021-00306-6
PII 10.1038/s41598-021-00306-6
PMID 34686700
PMC PMC8536758
MeSH Animals Cell Adhesion Molecules / metabolism Cell Differentiation / physiology* Cell Line Cell Line, Tumor HEK293 Cells Humans K562 Cells MAP Kinase Signaling System / physiology Muscle Development / physiology* Protein Binding / physiology Ubiquitin-Protein Ligases / metabolism* p38 Mitogen-Activated Protein Kinases / metabolism
IF 3.998
Human and Animal Cells C2C12(RCB0987)