論文 - 詳細
| RRC ID | 68559 |
|---|---|
| 著者 | Okumura F, Oki N, Fujiki Y, Ikuta R, Osaki K, Hamada S, Nakatsukasa K, Hisamoto N, Hara T, Kamura T. |
| タイトル | ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation. |
| ジャーナル | Sci Rep |
| Abstract |
Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor that mediates cell-cell interactions and positively regulates myogenesis. The cytoplasmic region of Cdon interacts with other proteins to form a Cdon/JLP/Bnip-2/CDC42 complex that activates p38 mitogen-activated protein kinase (MAPK) and induces myogenesis. However, Cdon complex may include other proteins during myogenesis. In this study, we found that Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) ubiquitin ligase is induced during C2C12 differentiation and is included in the Cdon complex. We knocked-down Zswim8 in C2C12 cells to determine the effect of ZSWIM8 on differentiation. However, we detected neither ZSWIM8-dependent ubiquitination nor the degradation of Bnip2, Cdon, or JLP. In contrast, ZSWIM8 knockdown accelerated C2C12 differentiation. These results suggest that ZSWIM8 is a Cdon complex-included myogenic protein that prevents C2C12 differentiation without affecting the stability of Bnip2, Cdon, and JLP. |
| 巻・号 | 11(1) |
| ページ | 20880 |
| 公開日 | 2021-10-22 |
| DOI | 10.1038/s41598-021-00306-6 |
| PII | 10.1038/s41598-021-00306-6 |
| PMID | 34686700 |
| PMC | PMC8536758 |
| MeSH | Animals Cell Adhesion Molecules / metabolism Cell Differentiation / physiology* Cell Line Cell Line, Tumor HEK293 Cells Humans K562 Cells MAP Kinase Signaling System / physiology Muscle Development / physiology* Protein Binding / physiology Ubiquitin-Protein Ligases / metabolism* p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |