| RRC ID |
68569
|
| 著者 |
Magny R, Kessal K, Regazzetti A, Ben Yedder A, Baudouin C, Mélik Parsadaniantz S, Brignole-Baudouin F, Laprévote O, Auzeil N.
|
| タイトル |
Lipidomic analysis of epithelial corneal cells following hyperosmolarity and benzalkonium chloride exposure: New insights in dry eye disease.
|
| ジャーナル |
Biochim Biophys Acta Mol Cell Biol Lipids
|
| Abstract |
Dry eye disease (DED) is a multifactorial chronic inflammatory disease of the ocular surface characterized by tear film instability, hyperosmolarity, cell damage and inflammation. Hyperosmolarity is strongly established as the core mechanism of the DED. Benzalkonium chloride (BAK) - a quaternary ammonium salt commonly used in eye drops for its microbicidal properties - is well known to favor the onset of DED. Currently, little data are available regarding lipid metabolism alteration in ocular surface epithelial cells in the course of DED. Our aim was to explore the effects of benzalkonium chloride or hyperosmolarity exposure on the human corneal epithelial (HCE) cell lipidome, two different conditions used as in vitro models of DED. For this purpose, we performed a lipidomic analysis using UPLC-HRMS-ESI+/-. Our results demonstrated that BAK or hyperosmolarity induced important modifications in HCE lipidome including major changes in sphingolipids, glycerolipids and glycerophospholipids. For both exposures, an increase in ceramide was especially exhibited. Hyperosmolarity specifically induced triglyceride accumulation resulting in lipid droplet formation. Conversely, BAK induced an increase in lysophospholipids and a decrease in phospholipids. This lipidomic study highlights the lipid changes involved in inflammatory responses following BAK or hyperosmolarity exposures. Thereby, lipid research appears of great interest, as it could lead to the discovery of new biomarkers and therapeutic targets for the diagnosis and treatment of dry eye disease.
|
| 巻・号 |
1865(9)
|
| ページ |
158728
|
| 公開日 |
2020-9-1
|
| DOI |
10.1016/j.bbalip.2020.158728
|
| PII |
S1388-1981(20)30120-7
|
| PMID |
32438057
|
| MeSH |
Benzalkonium Compounds / pharmacology*
Ceramides / metabolism
Cornea / cytology*
Cytokines / metabolism
Dry Eye Syndromes / metabolism
Epithelial Cells / drug effects*
Epithelial Cells / metabolism*
Lipid Droplets
Lipid Metabolism / drug effects*
Lipidomics
Osmolar Concentration*
Phospholipids / metabolism
Preservatives, Pharmaceutical / pharmacology*
Reactive Oxygen Species / metabolism
|
| IF |
4.519
|
| リソース情報 |
| ヒト・動物細胞 |
HCE-T(RCB2280) |
