| RRC ID |
68574
|
| 著者 |
Akiyama M, Yamada O, Kanda N, Akita S, Kawano T, Ohno T, Mizoguchi H, Eto Y, Anderson KC, Yamada H.
|
| タイトル |
Telomerase overexpression in K562 leukemia cells protects against apoptosis by serum deprivation and double-stranded DNA break inducing agents, but not against DNA synthesis inhibitors.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
Telomeres are specialized DNA/protein structures that act as protective caps to prevent end fusions. The maintenance of telomeres is essential for chromosomal stability. Telomerase is regulated by human telomerase reverse transcriptase (hTERT). c-Myc oncoprotein is also implicated in the positive regulation of hTERT expression. We show here that two clones of hTERT-transfected K562 erythroleukemia cells have elongated telomeres (22.5 and 24.0 kb), whereas telomere length of both c-Myc-transfected K562 cells and parental K562 cells is 6.5 kb. Telomerase activity and hTERT mRNA expression increased in hTERT-transfected K562 cells, while the expression levels of telomerase activity and hTERT in c-Myc-transfected K562 cells were similar to that in parental K562 cells, despite an overexpression of c-Myc. Importantly, we found that hTERT-transfected K562 cells are protected against apoptosis induced by serum deprivation and double-stranded DNA break inducing agents (ionizing irradiation, and etoposide (VP-16)), but not against DNA synthesis inhibitors (1-beta-D-arabinofuranosylcytosine and hydroxyurea). These findings suggest that overexpression of telomerase by transfecting hTERT confers telomere-elongation and resistance to double-stranded DNA break inducing agents.
|
| 巻・号 |
178(2)
|
| ページ |
187-97
|
| 公開日 |
2002-4-25
|
| DOI |
10.1016/s0304-3835(01)00838-2
|
| PII |
S0304383501008382
|
| PMID |
11867204
|
| MeSH |
Antineoplastic Agents / pharmacology
Apoptosis / drug effects
Apoptosis / genetics*
Apoptosis / radiation effects
Culture Media, Serum-Free
Cytarabine / pharmacology
DNA Damage / drug effects
DNA Damage / genetics
DNA Damage / radiation effects
DNA Replication / drug effects
Etoposide / pharmacology
Humans
Hydroxyurea / pharmacology
K562 Cells / drug effects
K562 Cells / enzymology*
K562 Cells / pathology*
K562 Cells / radiation effects
Nucleic Acid Synthesis Inhibitors / pharmacology
Telomerase / biosynthesis*
Telomerase / genetics
Telomere / pathology
Transfection
|
| IF |
7.36
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
