論文 - 詳細
| RRC ID | 68669 |
|---|---|
| 著者 | Kawai S, Amano A. |
| タイトル | BRCA1 regulates microRNA biogenesis via the DROSHA microprocessor complex. |
| ジャーナル | J Cell Biol |
| Abstract |
MicroRNAs (miRNAs) are noncoding RNAs that function as key posttranscriptional regulators of gene expression. miRNA maturation is controlled by the DROSHA microprocessor complex. However, the detailed mechanism of miRNA biogenesis remains unclear. We show that the tumor suppressor breast cancer 1 (BRCA1) accelerates the processing of miRNA primary transcripts. BRCA1 increased the expressions of both precursor and mature forms of let-7a-1, miR-16-1, miR-145, and miR-34a. In addition, this tumor suppressor was shown to be directly associated with DROSHA and DDX5 of the DROSHA microprocessor complex, and it interacted with Smad3, p53, and DHX9 RNA helicase. We also found that BRCA1 recognizes the RNA secondary structure and directly binds with primary transcripts of miRNAs via a DNA-binding domain. Together, these results suggest that BRCA1 regulates miRNA biogenesis via the DROSHA microprocessor complex and Smad3/p53/DHX9. Our findings also indicate novel functions of BRCA1 in miRNA biogenesis, which may be linked to its tumor suppressor mechanism and maintenance of genomic stability. |
| 巻・号 | 197(2) |
| ページ | 201-8 |
| 公開日 | 2012-4-16 |
| DOI | 10.1083/jcb.201110008 |
| PII | jcb.201110008 |
| PMID | 22492723 |
| PMC | PMC3328391 |
| MeSH | BRCA1 Protein / metabolism* Cell Line, Tumor DEAD-box RNA Helicases / metabolism DNA-Binding Proteins / metabolism Gene Expression Regulation HEK293 Cells HeLa Cells Humans MicroRNAs / biosynthesis* MicroRNAs / metabolism Neoplasm Proteins / metabolism RNA Interference RNA, Small Interfering Ribonuclease III / metabolism* Smad3 Protein / metabolism Tumor Suppressor Protein p53 / metabolism |
| IF | 8.811 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) 293(RCB1637) MG-63(RCB1890) |