論文 - 詳細
| RRC ID | 68754 |
|---|---|
| 著者 | Fort A, Hashimoto K, Yamada D, Salimullah M, Keya CA, Saxena A, Bonetti A, Voineagu I, Bertin N, Kratz A, Noro Y, Wong CH, de Hoon M, Andersson R, Sandelin A, Suzuki H, Wei CL, Koseki H, FANTOM Consortium, Hasegawa Y, Forrest AR, Carninci P. |
| タイトル | Deep transcriptome profiling of mammalian stem cells supports a regulatory role for retrotransposons in pluripotency maintenance. |
| ジャーナル | Nat Genet |
| Abstract |
The importance of microRNAs and long noncoding RNAs in the regulation of pluripotency has been documented; however, the noncoding components of stem cell gene networks remain largely unknown. Here we investigate the role of noncoding RNAs in the pluripotent state, with particular emphasis on nuclear and retrotransposon-derived transcripts. We have performed deep profiling of the nuclear and cytoplasmic transcriptomes of human and mouse stem cells, identifying a class of previously undetected stem cell-specific transcripts. We show that long terminal repeat (LTR)-derived transcripts contribute extensively to the complexity of the stem cell nuclear transcriptome. Some LTR-derived transcripts are associated with enhancer regions and are likely to be involved in the maintenance of pluripotency. |
| 巻・号 | 46(6) |
| ページ | 558-66 |
| 公開日 | 2014-6-1 |
| DOI | 10.1038/ng.2965 |
| PII | ng.2965 |
| PMID | 24777452 |
| MeSH | Animals Cell Nucleus / metabolism Chromatin / chemistry Cytoplasm / metabolism Enhancer Elements, Genetic Gene Expression Profiling Gene Regulatory Networks Humans Lymphocytes / cytology Mice MicroRNAs / metabolism Oligonucleotides, Antisense / genetics Pluripotent Stem Cells / cytology* Retroelements* Terminal Repeat Sequences Transcriptome* |
| IF | 27.605 |
| リソース情報 | |
| ヒト・動物細胞 | B6G-2(AES0003) FVB-1(AES0045) iPS-MEF-Ng-20D-17(APS0001) |