論文 - 詳細
| RRC ID | 68767 |
|---|---|
| 著者 | Yaguchi A, Oshikawa M, Watanabe G, Hiramatsu H, Uchida N, Hara C, Kaneko N, Sawamoto K, Muraoka T, Ajioka I. |
| タイトル | Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration. |
| ジャーナル | Nat Commun |
| Abstract |
During injured tissue regeneration, the extracellular matrix plays a key role in controlling and coordinating various cellular events by binding and releasing secreted proteins in addition to promoting cell adhesion. Herein, we develop a cell-adhesive fiber-forming peptide that mimics the jigsaw-shaped hydrophobic surface in the dovetail-packing motif of glycophorin A as an artificial extracellular matrix for regenerative therapy. We show that the jigsaw-shaped self-assembling peptide forms several-micrometer-long supramolecular nanofibers through a helix-to-strand transition to afford a hydrogel under physiological conditions and disperses homogeneously in the hydrogel. The molecular- and macro-scale supramolecular properties of the jigsaw-shaped self-assembling peptide hydrogel allow efficient incorporation and sustained release of vascular endothelial growth factor, and demonstrate cell transplantation-free regenerative therapeutic effects in a subacute-chronic phase mouse stroke model. This research highlights a therapeutic strategy for injured tissue regeneration using the jigsaw-shaped self-assembling peptide supramolecular hydrogel. |
| 巻・号 | 12(1) |
| ページ | 6623 |
| 公開日 | 2021-11-19 |
| DOI | 10.1038/s41467-021-26896-3 |
| PII | 10.1038/s41467-021-26896-3 |
| PMID | 34799548 |
| PMC | PMC8604910 |
| MeSH | Adhesives Animals Biomedical Engineering Brain Injuries / diagnostic imaging Brain Regeneration / physiology* Cell Adhesion Disease Models, Animal Female Green Fluorescent Proteins / chemistry Hydrogels / chemistry* Hydrogels / therapeutic use Hydrophobic and Hydrophilic Interactions Mice Mice, Inbred C57BL Nanofibers Nervous System Peptides / chemistry* Peptides / therapeutic use Proteins / chemistry* Vascular Endothelial Growth Factor A |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |