RRC ID 68767
Author Yaguchi A, Oshikawa M, Watanabe G, Hiramatsu H, Uchida N, Hara C, Kaneko N, Sawamoto K, Muraoka T, Ajioka I.
Title Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration.
Journal Nat Commun
Abstract During injured tissue regeneration, the extracellular matrix plays a key role in controlling and coordinating various cellular events by binding and releasing secreted proteins in addition to promoting cell adhesion. Herein, we develop a cell-adhesive fiber-forming peptide that mimics the jigsaw-shaped hydrophobic surface in the dovetail-packing motif of glycophorin A as an artificial extracellular matrix for regenerative therapy. We show that the jigsaw-shaped self-assembling peptide forms several-micrometer-long supramolecular nanofibers through a helix-to-strand transition to afford a hydrogel under physiological conditions and disperses homogeneously in the hydrogel. The molecular- and macro-scale supramolecular properties of the jigsaw-shaped self-assembling peptide hydrogel allow efficient incorporation and sustained release of vascular endothelial growth factor, and demonstrate cell transplantation-free regenerative therapeutic effects in a subacute-chronic phase mouse stroke model. This research highlights a therapeutic strategy for injured tissue regeneration using the jigsaw-shaped self-assembling peptide supramolecular hydrogel.
Volume 12(1)
Pages 6623
Published 2021-11-19
DOI 10.1038/s41467-021-26896-3
PII 10.1038/s41467-021-26896-3
PMID 34799548
PMC PMC8604910
MeSH Adhesives Animals Biomedical Engineering Brain Injuries / diagnostic imaging Brain Regeneration / physiology* Cell Adhesion Disease Models, Animal Female Green Fluorescent Proteins / chemistry Hydrogels / chemistry* Hydrogels / therapeutic use Hydrophobic and Hydrophilic Interactions Mice Mice, Inbred C57BL Nanofibers Nervous System Peptides / chemistry* Peptides / therapeutic use Proteins / chemistry* Vascular Endothelial Growth Factor A
IF 12.121
Human and Animal Cells 293T(RCB2202)