RRC ID 68786
Author Yoshida R, Nakayama H, Nagata M, Hirosue A, Tanaka T, Kawahara K, Nakagawa Y, Matsuoka Y, Sakata J, Arita H, Hiraki A, Shinohara M, Ito T.
Title Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma.
Journal Br J Cancer
Abstract BACKGROUND:Nucleostemin (NS) is essential for the maintenance of stem cell properties, the functions of which remain poorly understood in cancer cells. The purpose of this study was to explore the impact of NS on malignancy and its clinical significance in oral squamous cell carcinoma (OSCC) patients.
METHODS:We investigated the effects of NS on the proliferation and invasion of OSCC using NS-overexpressing or -knockdown OSCC cells. We assessed the activation of the STAT3 (signal transducer and activator of transcription 3) signalling pathway and the downstream targets in the cells with different expression levels of NS. An immunohistochemical analysis of NS was also performed in 54 OSCC patients who were treated with preoperative chemoradiotherapy and surgery.
RESULTS:The overexpression of NS significantly enhanced the proliferation and invasive potential of OSCC cells. On the other hand, downregulation of NS suppressed the invasiveness of the cells. The alterations of these malignant phenotypes were associated with the activation of STAT3 signalling and its downstream targets. An immunohistochemical analysis demonstrated that a high NS tumour expression level significantly correlated with an advanced T-stage and N-stage. Furthermore, a Cox regression analysis revealed that the NS status (hazard ratio, 9.09; P=0.002) was a significant progression factor for OSCC patients.
CONCLUSIONS:Our results suggest that targeting NS may provide a promising treatment for highly malignant OSCC.
Volume 111(12)
Pages 2308-15
Published 2014-12-9
DOI 10.1038/bjc.2014.539
PII bjc2014539
PMID 25314067
PMC PMC4264447
MeSH Biomarkers, Tumor / biosynthesis Biomarkers, Tumor / genetics Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Carcinoma, Squamous Cell / pathology Cell Proliferation / physiology GTP-Binding Proteins / biosynthesis* GTP-Binding Proteins / genetics Head and Neck Neoplasms / genetics Head and Neck Neoplasms / metabolism* Head and Neck Neoplasms / pathology Humans Immunohistochemistry Mouth Neoplasms / genetics Mouth Neoplasms / metabolism* Nuclear Proteins / biosynthesis* Nuclear Proteins / genetics Phenotype Prognosis Signal Transduction Squamous Cell Carcinoma of Head and Neck Transfection
IF 5.791
Human and Animal Cells SAS(RCB1974)