論文 - 詳細
| RRC ID | 68798 |
|---|---|
| 著者 | Yamashita H, Ichikawa T, Matsuyama D, Kimura Y, Ueda K, Craig SW, Harada I, Kioka N. |
| タイトル | The role of the interaction of the vinculin proline-rich linker region with vinexin α in sensing the stiffness of the extracellular matrix. |
| ジャーナル | J Cell Sci |
| Abstract |
Although extracellular matrix (ECM) stiffness is an important aspect of the extracellular microenvironment and is known to direct the lineage specification of stem cells and affect cancer progression, the molecular mechanisms that sense ECM stiffness have not yet been elucidated. In this study, we show that the proline-rich linker (PRL) region of vinculin and the PRL-region-binding protein vinexin are involved in sensing the stiffness of ECM substrates. A rigid substrate increases the level of cytoskeleton-associated vinculin, and the fraction of vinculin stably localizing at focal adhesions (FAs) is larger on rigid ECM than on soft ECM. Mutations in the PRL region or the depletion of vinexin expression impair these responses to ECM stiffness. Furthermore, vinexin depletion impairs the stiffness-dependent regulation of cell migration. These results suggest that the interaction of the PRL region of vinculin with vinexin α plays a crucial role in sensing ECM stiffness and in mechanotransduction. |
| 巻・号 | 127(Pt 9) |
| ページ | 1875-86 |
| 公開日 | 2014-5-1 |
| DOI | 10.1242/jcs.133645 |
| PII | jcs.133645 |
| PMID | 24554436 |
| MeSH | Animals Cells, Cultured Circular Dichroism Extracellular Matrix / metabolism* Fluorescence Recovery After Photobleaching Immunoprecipitation Mice Muscle Proteins / metabolism* Wound Healing / genetics Wound Healing / physiology |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |