RRC ID 68798
Author Yamashita H, Ichikawa T, Matsuyama D, Kimura Y, Ueda K, Craig SW, Harada I, Kioka N.
Title The role of the interaction of the vinculin proline-rich linker region with vinexin α in sensing the stiffness of the extracellular matrix.
Journal J Cell Sci
Abstract Although extracellular matrix (ECM) stiffness is an important aspect of the extracellular microenvironment and is known to direct the lineage specification of stem cells and affect cancer progression, the molecular mechanisms that sense ECM stiffness have not yet been elucidated. In this study, we show that the proline-rich linker (PRL) region of vinculin and the PRL-region-binding protein vinexin are involved in sensing the stiffness of ECM substrates. A rigid substrate increases the level of cytoskeleton-associated vinculin, and the fraction of vinculin stably localizing at focal adhesions (FAs) is larger on rigid ECM than on soft ECM. Mutations in the PRL region or the depletion of vinexin expression impair these responses to ECM stiffness. Furthermore, vinexin depletion impairs the stiffness-dependent regulation of cell migration. These results suggest that the interaction of the PRL region of vinculin with vinexin α plays a crucial role in sensing ECM stiffness and in mechanotransduction.
Volume 127(Pt 9)
Pages 1875-86
Published 2014-5-1
DOI 10.1242/jcs.133645
PII jcs.133645
PMID 24554436
MeSH Animals Cells, Cultured Circular Dichroism Extracellular Matrix / metabolism* Fluorescence Recovery After Photobleaching Immunoprecipitation Mice Muscle Proteins / metabolism* Wound Healing / genetics Wound Healing / physiology
Resource
Human and Animal Cells 293T(RCB2202)