論文 - 詳細
| RRC ID | 68921 |
|---|---|
| 著者 | Phan TKT, Do TL, Tachibana K, Kihara T. |
| タイトル | Alpha-mangostin dephosphorylates ERM to induce adhesion and decrease surface stiffness in KG-1 cells. |
| ジャーナル | Hum Cell |
| Abstract |
Surface stiffness is a unique indicator of various cellular states and events and needs to be tightly controlled. α-Mangostin, a natural compound with numerous bioactivities, reduces the mechanical stiffness of various cells; however, the mechanism by which it affects the actin cytoskeleton remains unclear. We aimed to elucidate the mechanism underlying α-mangostin activity on the surface stiffness of leukocytes. We treated spherical non-adherent myelomonocytic KG-1 cells with α-mangostin; it clearly reduced their surface stiffness and disrupted their microvilli. The α-mangostin-induced reduction in surface stiffness was inhibited by calyculin A, a protein phosphatase inhibitor. α-Mangostin also induced KG-1 cell adhesion to a fibronectin-coated surface. In KG-1 cells, a decrease in surface stiffness and the induction of cell adhesion are largely attributed to the dephosphorylation of ezrin/radixin/moesin proteins (ERMs); α-mangostin reduced the levels of phosphorylated ERMs. It further increased protein kinase C (PKC) activity. α-Mangostin-induced KG-1 cell adhesion and cell surface softness were inhibited by the PKC inhibitor GF109203X. The results of the present study suggest that α-mangostin decreases stiffness and induces adhesion of KG-1 cells via PKC activation and ERM dephosphorylation. |
| 巻・号 | 35(1) |
| ページ | 189-198 |
| 公開日 | 2022-1-1 |
| DOI | 10.1007/s13577-021-00651-8 |
| PII | 10.1007/s13577-021-00651-8 |
| PMID | 34817798 |
| MeSH | Cell Adhesion / drug effects* Cells, Cultured Cytoskeletal Proteins / metabolism* Elasticity / drug effects* Enzyme Inhibitors / pharmacology Humans Indoles / pharmacology Maleimides / pharmacology Membrane Proteins / metabolism* Microfilament Proteins / metabolism* Phosphorylation / drug effects Protein Kinase C / antagonists & inhibitors Protein Kinase C / metabolism Xanthones / pharmacology* |
| IF | 3.463 |
| リソース情報 | |
| ヒト・動物細胞 | KG-1(RCB1166) U-937 DE-4(RCB0435) |