RRC ID 68925
著者 Harada K, Ferdous T, Mishima K.
タイトル Efficacy of a Novel Oral Chemotherapeutic Agent, TAS-102, Against Human Oral Squamous Cell Carcinoma Cells.
ジャーナル Anticancer Res
Abstract BACKGROUND:TAS-102 is effective against unresectable advanced or recurrent colorectal and gastric cancer. However, its effect on oral squamous cell carcinoma (OSCC) is still unknown. Here, we tried to clarify the possible effect of TAS-102 against angiogenesis and proliferation of human OSCC cells.
MATERIALS AND METHODS:3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, migration assay and mice xenograft models were used to determine the effect of TAS-102 on growth and migration of OSCC. The activity of phosphorylated nuclear factor kappa light-chain-enhancer of activated B-cells (NF-κB) (p-p65) in cells was detected by immunocytochemistry. The expression of p-AKT serine/threonine kinase 1 (p-AKT), p-p65, vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF2) and CD31 in mouse tumors were detected by immunohistochemistry.
RESULTS:TAS-102 significantly inhibited growth and migration of OSCC both in vitro and in vivo. It suppressed the activity of NF-κB in cells. TAS-102 down-regulated the expression of p-AKT, VEGF, FGF2 and CD31, which was associated with reduced vascularization of HSC2 tumor lesions.
CONCLUSION:These findings suggest that TAS-102 might inhibit angiogenesis and proliferation of OSCC cells.
巻・号 41(12)
ページ 6039-6049
公開日 2021-12-1
DOI 10.21873/anticanres.15423
PII 41/12/6039
PMID 34848458
MeSH Animals Antineoplastic Agents / administration & dosage Antineoplastic Agents / pharmacology* Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Disease Models, Animal Drug Combinations Female Gene Expression Regulation, Neoplastic / drug effects Humans Mice Mouth Neoplasms NF-kappa B / metabolism Neovascularization, Pathologic / drug therapy Pyrrolidines / administration & dosage Pyrrolidines / pharmacology* Signal Transduction Squamous Cell Carcinoma of Head and Neck Thymine / administration & dosage Thymine / pharmacology* Trifluridine / administration & dosage Trifluridine / pharmacology* Xenograft Model Antitumor Assays
IF 1.994
リソース情報
ヒト・動物細胞 HSC-2(RCB1945) HSC-4(RCB1902)