RRC ID 690
Author Nemoto Y, Kisaka M, Fuse T, Yano M, Ogihara Y.
Title Characterization and functional analysis of three wheat genes with homology to the CONSTANS flowering time gene in transgenic rice.
Journal Plant J
Abstract The CONSTANS (CO) gene of Arabidopsis plays a key role in the photoperiodic flowering pathway. To investigate photoperiod responses in cereals in more detail, we isolated three kinds of CO/Hd1 (rice ortholog of CO) homolog from hexaploid wheat, derived from the A, B, and D genomes and designated as wheat ortholog of CO from A genome (TaHd1-1), TaHd1-2, and TaHd1-3, respectively. They were highly similar to each other and to Hd1, and in addition harbored two conserved regions: two zinc finger motifs and CONSTANS, CONSTANS-LIKE and TIMING OF CAB EXPRESSION 1 (CCT) domain like CO/Hd1. They were located on the long arm of the homoeologous chromosome 6. TaHd1-2 harbored a 63-bp deletion at the promoter region containing the GATA-1 box, and consequently, we detected no subsequent transcript. The TaHd1-1 genomic clone was introduced to a rice line deficient in Hd1 function. Transgenic plants complemented the functions of rice Hd1: they promoted heading under short-day (SD) conditions and delayed it under long-day (LD)/natural conditions, indicating that Hd1 proteins from SD and LD plants share common structures and functions.
Volume 36(1)
Pages 82-93
Published 2003-10-1
DOI 10.1046/j.1365-313x.2003.01859.x
PII 1859
PMID 12974813
MeSH Amino Acid Sequence Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Chromosome Mapping Cloning, Molecular DNA, Complementary / chemistry DNA, Complementary / genetics DNA, Plant / chemistry DNA, Plant / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Flowers / genetics Flowers / growth & development* Gene Expression Profiling Genetic Complementation Test Molecular Sequence Data Oryza / genetics Oryza / growth & development* Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism Triticum / genetics* Triticum / growth & development
IF 6.141
Times Cited 104
Wheat CS TAC library