RRC ID 69050
Author Inami S, Sato T, Kurata Y, Suzuki Y, Kitamoto T, Sakai T.
Title Consolidation and maintenance of long-term memory involve dual functions of the developmental regulator Apterous in clock neurons and mushroom bodies in the Drosophila brain.
Journal PLoS Biol
Abstract Memory is initially labile but can be consolidated into stable long-term memory (LTM) that is stored in the brain for extended periods. Despite recent progress, the molecular and cellular mechanisms underlying the intriguing neurobiological processes of LTM remain incompletely understood. Using the Drosophila courtship conditioning assay as a memory paradigm, here, we show that the LIM homeodomain (LIM-HD) transcription factor Apterous (Ap), which is known to regulate various developmental events, is required for both the consolidation and maintenance of LTM. Interestingly, Ap is involved in these 2 memory processes through distinct mechanisms in different neuronal subsets in the adult brain. Ap and its cofactor Chip (Chi) are indispensable for LTM maintenance in the Drosophila memory center, the mushroom bodies (MBs). On the other hand, Ap plays a crucial role in memory consolidation in a Chi-independent manner in pigment dispersing factor (Pdf)-containing large ventral-lateral clock neurons (l-LNvs) that modulate behavioral arousal and sleep. Since disrupted neurotransmission and electrical silencing in clock neurons impair memory consolidation, Ap is suggested to contribute to the stabilization of memory by ensuring the excitability of l-LNvs. Indeed, ex vivo imaging revealed that a reduced function of Ap, but not Chi, results in exaggerated Cl- responses to the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) in l-LNvs, indicating that wild-type (WT) Ap maintains high l-LNv excitability by suppressing the GABA response. Consistently, enhancing the excitability of l-LNvs by knocking down GABAA receptors compensates for the impaired memory consolidation in ap null mutants. Overall, our results revealed unique dual functions of the developmental regulator Ap for LTM consolidation in clock neurons and LTM maintenance in MBs.
Volume 19(12)
Pages e3001459
Published 2021-12-1
DOI 10.1371/journal.pbio.3001459
PMID 34860826
PMC PMC8641882
MeSH Animals Biological Clocks / physiology* Brain / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / physiology* Heterozygote LIM-Homeodomain Proteins / genetics LIM-Homeodomain Proteins / metabolism* Memory Consolidation / physiology* Memory, Long-Term / physiology* Models, Biological Mushroom Bodies / physiology* Mutation / genetics Neurons / physiology* Phenotype Synaptic Transmission / physiology Transcription Factors / genetics Transcription Factors / metabolism* gamma-Aminobutyric Acid / pharmacology
IF 7.076
Drosophila 8376R-1