論文 - 詳細
| RRC ID | 69187 |
|---|---|
| 著者 | Nagano T, Hashimoto T, Nakashima A, Hisanaga S, Kikkawa U, Kamada S. |
| タイトル | Cyclin I is involved in the regulation of cell cycle progression. |
| ジャーナル | Cell Cycle |
| Abstract |
Cyclins control cell cycle progression by regulating the activity of cyclin-dependent kinases (Cdks). Cyclin I is a member of the cyclin family because of the presence of a cyclin box motif. It has been suggested that Cyclin I is involved in various biological processes, such as cell survival, angiogenesis, and cell differentiation. However, whether or not Cyclin I has a role in regulating the cell cycle similarly to other cyclins has yet to be clarified. Therefore, we investigated the role for Cyclin I in cell cycle progression. We showed that the protein level of Cyclin I oscillated during the cell cycle, and that Cyclin I was subjected to ubiquitination and degradation in cells. The interaction between Cyclin I and Cdk5 was detected in cells overexpressed with both proteins. Furthermore, depletion of Cyclin I by siRNAs prevented cell proliferation, suggesting the positive role of Cyclin I for the cell cycle progression. In addition, flow cytometric analysis revealed that cells depleted of Cyclin I were accumulated at G₂/M phases. By using HeLa.S-Fucci (fluorescent ubiquitination-based cell cycle indicator) cells, we further confirmed that knockdown of Cyclin I induced cell cycle arrest at S/G₂/M phases. These results strongly suggest that Cyclin I has the role in the regulation of cell cycle progression. |
| 巻・号 | 12(16) |
| ページ | 2617-24 |
| 公開日 | 2013-8-15 |
| DOI | 10.4161/cc.25623 |
| PII | 25623 |
| PMID | 23907122 |
| PMC | PMC3865051 |
| MeSH | Cell Cycle / physiology* Cell Proliferation Cyclin I / genetics Cyclin I / metabolism* Cyclin-Dependent Kinase 5 / metabolism Cyclin-Dependent Kinases / metabolism* Flow Cytometry Fluorescence Gene Knockdown Techniques HeLa Cells Humans Proteolysis Ubiquitination |
| IF | 3.699 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |