RRC ID 69591
Author Kase-Kato I, Asai S, Minemura C, Tsuneizumi K, Oshima S, Koma A, Kasamatsu A, Hanazawa T, Uzawa K, Seki N.
Title Molecular Pathogenesis of the Coronin Family: CORO2A Facilitates Migration and Invasion Abilities in Oral Squamous Cell Carcinoma.
Journal Int J Mol Sci
Abstract In humans, the coronin family is composed of seven proteins containing WD-repeat domains that regulate actin-based cellular processes. Some members of the coronin family are closely associated with cancer cell migration and invasion. The Cancer Genome Atlas (TCGA) analysis revealed that CORO1C, CORO2A, and CORO7 were significantly upregulated in oral squamous cell carcinoma (OSCC) tissues (p < 0.05). Moreover, the high expression of CORO2A was significantly predictive of the 5-year survival rate of patients with OSCC (p = 0.0203). Overexpression of CORO2A was detected in OSCC clinical specimens by immunostaining. siRNA-mediated knockdown of CORO2A suppressed cancer cell migration and invasion abilities. Furthermore, we investigated the involvement of microRNAs (miRNAs) in the molecular mechanism underlying CORO2A overexpression in OSCC cells. TCGA analysis confirmed that tumor-suppressive miR-125b-5p and miR-140-5p were significantly downregulated in OSCC tissues. Notably, these miRNAs bound directly to the 3'-UTR of CORO2A and controlled CORO2A expression in OSCC cells. In summary, we found that aberrant expression of CORO2A facilitates the malignant transformation of OSCC cells, and that downregulation of tumor-suppressive miRNAs is involved in CORO2A overexpression. Elucidation of the interaction between genes and miRNAs will help reveal the molecular pathogenesis of OSCC.
Volume 22(23)
Published 2021-11-24
DOI 10.3390/ijms222312684
PII ijms222312684
PMID 34884487
PMC PMC8657730
MeSH Apoptosis Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism Carcinoma, Squamous Cell / pathology* Cell Movement* Cell Proliferation Gene Expression Regulation, Neoplastic* Humans MicroRNAs / genetics* Microfilament Proteins / genetics Microfilament Proteins / metabolism* Mouth Neoplasms / genetics Mouth Neoplasms / metabolism Mouth Neoplasms / pathology* Neoplasm Invasiveness Prognosis Survival Rate Tumor Cells, Cultured
IF 4.556
Human and Animal Cells MRC-5 Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) Sa3(RCB0980) SAS(RCB1974)