論文 - 詳細
| RRC ID | 69754 |
|---|---|
| 著者 | Suzuki-Karasaki M, Ando T, Ochiai Y, Kawahara K, Suzuki-Karasaki M, Nakayama H, Suzuki-Karasaki Y. |
| タイトル | Air Plasma-Activated Medium Evokes a Death-Associated Perinuclear Mitochondrial Clustering. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Intractable cancers such as osteosarcoma (OS) and oral cancer (OC) are highly refractory, recurrent, and metastatic once developed, and their prognosis is still disappointing. Tumor-targeted therapy, which eliminates cancers effectively and safely, is the current clinical choice. Since aggressive tumors are substantially resistant to multidisciplinary therapies that target apoptosis, tumor-specific activation of another cell death modality is a promising avenue for meeting this goal. Here, we report that a cold atmospheric air plasma-activated medium (APAM) can kill OS and OC by causing a unique mitochondrial clustering. This event was named monopolar perinuclear mitochondrial clustering (MPMC) based on its characteristic unipolar mitochondrial perinuclear accumulation. The APAM caused apoptotic and nonapoptotic cell death. The APAM increased mitochondrial ROS (mROS) and cell death, and the antioxidants such as N-acetylcysteine (NAC) prevented them. MPMC occurred following mitochondrial fragmentation, which coincided with nuclear damages. MPMC was accompanied by mitochondrial lipid peroxide (mLPO) accumulation and prevented by NAC, Ferrostatin-1, and Nocodazole. In contrast, the APAM induced minimal cell death, mROS generation, mLPO accumulation, and MPMC in fibroblasts. These results suggest that MPMC occurs in a tumor-specific manner via mitochondrial oxidative stress and microtubule-driven mitochondrial motility. MPMC induction might serve as a promising target for exerting tumor-specific cytotoxicity. |
| 巻・号 | 23(3) |
| 公開日 | 2022-1-20 |
| DOI | 10.3390/ijms23031124 |
| PII | ijms23031124 |
| PMID | 35163042 |
| PMC | PMC8835529 |
| MeSH | Animals Bone Neoplasms / drug therapy* Bone Neoplasms / metabolism Cell Death Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Cluster Analysis Humans Lipid Peroxides / metabolism Male Mice Mitochondria / drug effects Mitochondria / metabolism* Mitochondrial Dynamics / drug effects Mouth Neoplasms / drug therapy* Mouth Neoplasms / metabolism Osteosarcoma / drug therapy* Osteosarcoma / metabolism Plasma Gases / administration & dosage* Plasma Gases / pharmacology Reactive Oxygen Species / metabolism Xenograft Model Antitumor Assays |
| IF | 4.556 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HOS(RCB0992) 143B/TK^(-)neo^(R)(RCB0701) LM8(RCB1450) |