論文 - 詳細
| RRC ID | 69791 |
|---|---|
| 著者 | Achudhan D, Liu SC, Lin YY, Huang CC, Tsai CH, Ko CY, Chiang IP, Kuo YH, Tang CH. |
| タイトル | Antcin K Inhibits TNF-α, IL-1β and IL-8 Expression in Synovial Fibroblasts and Ameliorates Cartilage Degradation: Implications for the Treatment of Rheumatoid Arthritis. |
| ジャーナル | Front Immunol |
| Abstract |
Extracts from Taiwan's traditional medicinal mushroom, Antrodia cinnamomea, exhibit anti-inflammatory activities in cellular and preclinical studies. However, this paper is the first to report that Antcin K, a triterpenoid isolated from A. cinnamomea, inhibits proinflammatory cytokine production in human rheumatoid synovial fibroblasts (RASFs), which are major players in rheumatoid arthritis (RA) disease. In our analysis of the mechanism of action, Antcin K inhibited the expression of three cytokines (tumor necrosis factor alpha [TNF-α], interleukin 1 beta [IL-1β] and IL-8) in human RASFs; cytokines that are crucial to RA synovial inflammation. Notably, incubation of RASFs with Antcin K reduced the phosphorylation of the focal adhesion kinase (FAK), phosphoinositide 3-kinase (PI3K), protein kinase B (AKT) and nuclear factor-κB (NF-κB) signaling cascades, all of which promote cytokine production in RA. Intraperitoneal injections of Antcin K (10 mg/kg or 30 mg/kg) attenuated paw swelling, cartilage degradation and bone erosion, and decreased serum levels of TNF-α, IL-1β, IL-8 in collagen-induced arthritis (CIA) mice; in further experiments, IL-6 levels were similarly reduced. The inhibitory effects of Antcin K upon TNF-α, IL-1β and IL-8 expression in human RASFs was achieved through the downregulation of the FAK, PI3K, AKT and NF-κB signaling cascades. Our data support clinical investigations using Antcin K in RA disease. |
| 巻・号 | 12 |
| ページ | 790925 |
| 公開日 | 2021-1-1 |
| DOI | 10.3389/fimmu.2021.790925 |
| PMID | 34975889 |
| PMC | PMC8714747 |
| MeSH | Animals Arthritis, Rheumatoid / drug therapy* Cartilage / metabolism* Cells, Cultured Cholestenes / pharmacology* Cholestenes / therapeutic use Cytokines / antagonists & inhibitors* Cytokines / biosynthesis Fibroblasts / drug effects Fibroblasts / immunology Humans Interleukin-1beta / antagonists & inhibitors Interleukin-8 / antagonists & inhibitors Mice Mice, Inbred C57BL NF-kappa B / physiology Phosphatidylinositol 3-Kinases / physiology Proto-Oncogene Proteins c-akt / physiology Synovial Membrane / drug effects* Synovial Membrane / immunology Tumor Necrosis Factor-alpha / antagonists & inhibitors |
| IF | 5.085 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |