論文 - 詳細
| RRC ID | 69947 |
|---|---|
| 著者 | Haraguchi T, Koujin T, Shindo T, Bilir Ş, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y. |
| タイトル | Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase. |
| ジャーナル | Commun Biol |
| Abstract |
DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe transfection tools. However, they have no effect on the transfection of non-proliferating cells, the reason for which is not well understood. This study elucidates the mechanism through which transfected DNA enters the nucleus for gene expression. To monitor the behavior of transfected DNA, we introduce plasmid bearing lacO repeats and RFP-coding sequences into cells expressing GFP-LacI and observe plasmid behavior and RFP expression in living cells. RFP expression appears only after mitosis. Electron microscopy reveals that plasmids are wrapped with nuclear envelope (NE)‒like membranes or associated with chromosomes at telophase. The depletion of BAF, which is involved in NE reformation, delays plasmid RFP expression. These results suggest that transfected DNA is incorporated into the nucleus during NE reformation at telophase. |
| 巻・号 | 5(1) |
| ページ | 78 |
| 公開日 | 2022-1-20 |
| DOI | 10.1038/s42003-022-03021-8 |
| PII | 10.1038/s42003-022-03021-8 |
| PMID | 35058555 |
| PMC | PMC8776997 |
| MeSH | Biological Transport Cell Line, Tumor Cell Nucleus / physiology* DNA / genetics* Gene Expression Regulation / physiology* Humans Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism* Plasmids / genetics* Single-Cell Analysis Telophase Transfection |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1224 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |