| RRC ID |
69991
|
| 著者 |
Wang ZF, Mi TW, Gao YQ, Feng HQ, Wu WH, Wang Y.
|
| タイトル |
STOP1 Regulates LKS1 Transcription and Coordinates K+/NH4+ Balance in Arabidopsis Response to Low-K+ Stress.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Potassium and nitrogen are essential mineral elements for plant growth and development. The protein kinase LKS1/CIPK23 is involved in both K+ and NH4+ uptake in Arabidopsis root. The transcripts of LKS1 can be induced by low K+ (0.1 mM) and high NH4+ (30 mM); however, the molecular mechanism is still unknown. In this study, we isolated the transcription factor STOP1 that positively regulates LKS1 transcription in Arabidopsis responses to both low-K+ and high-NH4+ stresses. STOP1 proteins can directly bind to the LKS1 promoter, promoting its transcription. The stop1 mutants displayed a leaf chlorosis phenotype similar to lks1 mutant when grown on low-K+ and high-NH4+ medium. On the other hand, STOP1 overexpressing plants exhibited a similar tolerant phenotype to LKS1 overexpressing plants. The transcript level of STOP1 was only upregulated by low K+ rather than high NH4+; however, the accumulation of STOP1 protein in the nucleus was required for the upregulation of LKS1 transcripts in both low-K+ and high-NH4+ responses. Our data demonstrate that STOP1 positively regulates LKS1 transcription under low-K+ and high-NH4+ conditions; therefore, LKS1 promotes K+ uptake and inhibits NH4+ uptake. The STOP1/LKS1 pathway plays crucial roles in K+ and NH4+ homeostasis, which coordinates potassium and nitrogen balance in plants in response to external fluctuating nutrient levels.
|
| 巻・号 |
23(1)
|
| 公開日 |
2021-12-29
|
| DOI |
10.3390/ijms23010383
|
| PII |
ijms23010383
|
| PMID |
35008809
|
| PMC |
PMC8745191
|
| MeSH |
Ammonium Compounds / metabolism*
Arabidopsis / drug effects
Arabidopsis / genetics*
Arabidopsis / physiology*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Base Sequence
Gene Expression Regulation, Plant
Genes, Plant
Models, Biological
Mutation / genetics
Plant Roots / metabolism
Potassium / metabolism*
Potassium / pharmacology
Promoter Regions, Genetic / genetics
Protein Binding / drug effects
Protoplasts / drug effects
Protoplasts / metabolism
Stress, Physiological* / drug effects
Stress, Physiological* / genetics
Transcription Factors / metabolism*
Transcription, Genetic* / drug effects
|
| IF |
4.556
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
psi00011
psi00012 |
