論文 - 詳細
| RRC ID | 70118 |
|---|---|
| 著者 | Nozaki M, Nishizuka M. |
| タイトル | Repression of RhoJ expression promotes TGF-β-mediated EMT in human non-small-cell lung cancer A549cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Non-small-cell lung cancer (NSCLC) accounts for most cancer-related deaths because of its strong metastatic ability. It is important to understand NSCLC's molecular mechanisms of metastasis. RhoJ, a protein that belongs to the Rho family of small GTPases, regulates endothelial motility, angiogenesis, and adipogenesis. Recently, bioinformatics analysis showed that NSCLC patients with lower RhoJ expression had a worse survival outcome than those with high RhoJ expression. However, little is known about RhoJ's role in NSCLC. In the present study, we demonstrated that RhoJ knockdown accelerated TGF-βmediated epithelial-to-mesenchymal transition (EMT), an important cancer metastasis process, in A549 and PC-9 cells. Furthermore, using Matrigel-coated transwell chambers, we showed that RhoJ knockdown enhanced the invasion capacity of A549 cells that had undergone EMT. Also, reduced RhoJ expression increased Smad3 phosphorylation and Snail expression during the EMT process. Our results provide the first evidence of a potential novel role for RhoJ in the inhibition of EMT via modulation of the TGF-β-Smad signaling pathway, and shed new light on the mechanisms underlying EMT in NSCLC. |
| 巻・号 | 566 |
| ページ | 94-100 |
| 公開日 | 2021-8-20 |
| DOI | 10.1016/j.bbrc.2021.06.004 |
| PII | S0006-291X(21)00919-0 |
| PMID | 34119829 |
| MeSH | A549 Cells Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / metabolism Carcinoma, Non-Small-Cell Lung / pathology* Down-Regulation Epithelial-Mesenchymal Transition* Gene Expression Regulation, Neoplastic Humans Lung Neoplasms / genetics Lung Neoplasms / metabolism Lung Neoplasms / pathology* Neoplasm Invasiveness / genetics Neoplasm Invasiveness / pathology Transforming Growth Factor beta / metabolism* rho GTP-Binding Proteins / genetics* rho GTP-Binding Proteins / metabolism |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 PC-9(RCB4455) |