Reference - Detail
| RRC ID | 70264 |
|---|---|
| Author | Itaya M, Omori A, Kanaya S, Crouch RJ, Tanaka T, Kondo K. |
| Title | Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. |
| Journal | J Bacteriol |
| Abstract |
Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were obtained from a B. subtilis DNA library. One (28.2 kDa) revealed high similarity to Escherichia coli RNase HII, encoded by the rnhB gene. The other (33.9 kDa) was designated rnhC and encodes B. subtilis RNase HIII. The B. subtilis genome has an rnhA homologue, the product of which has not yet shown RNase H activity. Analyses of all three B. subtilis genes revealed that rnhB and rnhC cannot be simultaneously inactivated. This observation indicated that in B. subtilis both the rnhB and rnhC products are involved in certain essential cellular processes that are different from those suggested by E. coli rnh mutation studies. Sequence conservation between the rnhB and rnhC genes implies that both originated from a single ancestral RNase H gene. The roles of bacterial RNase H may be indicated by the single rnhC homologue in the small genome of Mycoplasma species. |
| Volume | 181(7) |
| Pages | 2118-23 |
| Published | 1999-4-1 |
| DOI | 10.1128/JB.181.7.2118-2123.1999 |
| PMID | 10094689 |
| PMC | PMC93624 |
| MeSH | Bacillus subtilis / enzymology* Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacterial Proteins* Cloning, Molecular Conserved Sequence Databases, Factual Genes, Bacterial* Genome, Bacterial Mutagenesis Ribonuclease H / genetics* Ribonucleases* Sequence Analysis Sequence Homology, Amino Acid |
| IF | 3.006 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes B. subtilis | MBS952 MBS953 MBS954 MBS955 MBS956 |