| RRC ID |
70264
|
| 著者 |
Itaya M, Omori A, Kanaya S, Crouch RJ, Tanaka T, Kondo K.
|
| タイトル |
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were obtained from a B. subtilis DNA library. One (28.2 kDa) revealed high similarity to Escherichia coli RNase HII, encoded by the rnhB gene. The other (33.9 kDa) was designated rnhC and encodes B. subtilis RNase HIII. The B. subtilis genome has an rnhA homologue, the product of which has not yet shown RNase H activity. Analyses of all three B. subtilis genes revealed that rnhB and rnhC cannot be simultaneously inactivated. This observation indicated that in B. subtilis both the rnhB and rnhC products are involved in certain essential cellular processes that are different from those suggested by E. coli rnh mutation studies. Sequence conservation between the rnhB and rnhC genes implies that both originated from a single ancestral RNase H gene. The roles of bacterial RNase H may be indicated by the single rnhC homologue in the small genome of Mycoplasma species.
|
| 巻・号 |
181(7)
|
| ページ |
2118-23
|
| 公開日 |
1999-4-1
|
| DOI |
10.1128/JB.181.7.2118-2123.1999
|
| PMID |
10094689
|
| PMC |
PMC93624
|
| MeSH |
Bacillus subtilis / enzymology*
Bacillus subtilis / genetics*
Bacillus subtilis / growth & development
Bacterial Proteins*
Cloning, Molecular
Conserved Sequence
Databases, Factual
Genes, Bacterial*
Genome, Bacterial
Mutagenesis
Ribonuclease H / genetics*
Ribonucleases*
Sequence Analysis
Sequence Homology, Amino Acid
|
| IF |
3.006
|
| リソース情報 |
| 原核生物(枯草菌) |
MBS952
MBS953
MBS954
MBS955
MBS956 |
