論文 - 詳細
| RRC ID | 70265 |
|---|---|
| 著者 | Itaya M, Nagasaku M, Shimada T, Ohtani N, Shiwa Y, Yoshikawa H, Kaneko S, Tomita M, Sato M. |
| タイトル | Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Bacillus subtilis (natto) is generally regarded as a safe bacterium and used as a host for the production of several materials. However, genetic engineering of B. subtilis (natto) is not well established because of poor DNA delivery methods and the lack of a standard strain for the aim. Here, we developed a genetic delivery tool in B. subtilis (natto) using the pLS20 conjugational plasmid (65 kbp). Transmission of pLS20 from B. subtilis 168 to wild-type B. subtilis (natto) did not occur via established mating protocols. We isolated B. subtilis (natto) mutants showing dramatically increased recipient activity. Whole-genome sequence analyses revealed three common alterations: mutations in the restriction endonuclease gene and in the methyl-accepting chemotaxis protein gene, and a 43-kbp deletion at the genome replication termination locus. A representative strain named NEST116 was generated as the first B. subtilis (natto) strain suitable for exploring pLS20-based genetic engineering. |
| 巻・号 | 366(4) |
| 公開日 | 2019-2-1 |
| DOI | 10.1093/femsle/fnz032 |
| PII | 5307882 |
| PMID | 30726909 |
| MeSH | Bacillus subtilis / genetics* Conjugation, Genetic DNA, Bacterial / genetics* Gene Transfer Techniques* Genetic Engineering / methods* Mutation Plasmids / genetics* |
| IF | 1.987 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS937 MBS938 MBS939 MBS940 MBS941 MBS942 MBS943 |