RRC ID 704
著者 Nasuda S, Hudakova S, Schubert I, Houben A, Endo TR.
タイトル Stable barley chromosomes without centromeric repeats.
ジャーナル Proc Natl Acad Sci U S A
Abstract The satellite sequences (AGGGAG)(n) and Ty3/gypsy-like retrotransposons are known to localize at the barley centromeres. Using a gametocidal system, which induces chromosomal mutations in barley chromosomes added to common wheat, we obtained an isochromosome for the short arm of barley chromosome 7H (7HS) that lacked the barley-specific satellite sequence (AGGGAG)(n). Two telocentric derivatives of the isochromosome arose in the progeny: 7HS* with and 7HS** without the pericentromeric C-band. FISH analysis demonstrated that both telosomes lacked not only the barley-specific centromeric (AGGGAG)(n) repeats and retroelements but also any of the known wheat centromeric tandem repeats, including the 192-bp, 250-bp, and TaiI sequences. Although they lacked these centromeric repeats, 7HS* and 7HS** both showed normal mitotic and meiotic transmission. Translocation of barley centromeric repeats to a wheat chromosome 4A did not generate a dicentric chromosome. Indirect immunostaining revealed that all tested centromere-specific proteins (rice CENH3, maize CENP-C, and putative barley homologues of the yeast kinetochore proteins CBF5 and SKP1) and histone H3 phosphorylated at serines 10 and 28 localized at the centromeric region of 7HS*. We conclude that the barley centromeric repeats are neither sufficient nor obligatory to assemble kinetochores, and we discuss the possible formation of a novel centromere in a barley chromosome.
巻・号 102(28)
ページ 9842-7
公開日 2005-7-12
DOI 10.1073/pnas.0504235102
PII 0504235102
PMID 15998740
PMC PMC1175009
MeSH Azure Stains Centromere / genetics* Centromere / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosomes, Plant / genetics* Fluorescent Antibody Technique, Indirect Gene Transfer Techniques Histones / metabolism Hordeum / genetics* In Situ Hybridization, Fluorescence Isochromosomes / genetics Kinetochores / metabolism Repetitive Sequences, Nucleic Acid / genetics* Retroelements / genetics* Triticum / genetics
IF 9.412
引用数 159
WOS 分野 GENETICS & HEREDITY
リソース情報
コムギ LPGKU lines