論文 - 詳細
| RRC ID | 704 |
|---|---|
| 著者 | Nasuda S, Hudakova S, Schubert I, Houben A, Endo TR. |
| タイトル | Stable barley chromosomes without centromeric repeats. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
The satellite sequences (AGGGAG)(n) and Ty3/gypsy-like retrotransposons are known to localize at the barley centromeres. Using a gametocidal system, which induces chromosomal mutations in barley chromosomes added to common wheat, we obtained an isochromosome for the short arm of barley chromosome 7H (7HS) that lacked the barley-specific satellite sequence (AGGGAG)(n). Two telocentric derivatives of the isochromosome arose in the progeny: 7HS* with and 7HS** without the pericentromeric C-band. FISH analysis demonstrated that both telosomes lacked not only the barley-specific centromeric (AGGGAG)(n) repeats and retroelements but also any of the known wheat centromeric tandem repeats, including the 192-bp, 250-bp, and TaiI sequences. Although they lacked these centromeric repeats, 7HS* and 7HS** both showed normal mitotic and meiotic transmission. Translocation of barley centromeric repeats to a wheat chromosome 4A did not generate a dicentric chromosome. Indirect immunostaining revealed that all tested centromere-specific proteins (rice CENH3, maize CENP-C, and putative barley homologues of the yeast kinetochore proteins CBF5 and SKP1) and histone H3 phosphorylated at serines 10 and 28 localized at the centromeric region of 7HS*. We conclude that the barley centromeric repeats are neither sufficient nor obligatory to assemble kinetochores, and we discuss the possible formation of a novel centromere in a barley chromosome. |
| 巻・号 | 102(28) |
| ページ | 9842-7 |
| 公開日 | 2005-7-12 |
| DOI | 10.1073/pnas.0504235102 |
| PII | 0504235102 |
| PMID | 15998740 |
| PMC | PMC1175009 |
| MeSH | Azure Stains Centromere / genetics* Centromere / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosomes, Plant / genetics* Fluorescent Antibody Technique, Indirect Gene Transfer Techniques Histones / metabolism Hordeum / genetics* In Situ Hybridization, Fluorescence Isochromosomes / genetics Kinetochores / metabolism Repetitive Sequences, Nucleic Acid / genetics* Retroelements / genetics* Triticum / genetics |
| IF | 9.412 |
| 引用数 | 159 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| コムギ | LPGKU lines |