| RRC ID |
70526
|
| Author |
Burmeister AR, Fortier A, Roush C, Lessing AJ, Bender RG, Barahman R, Grant R, Chan BK, Turner PE.
|
| Title |
Pleiotropy complicates a trade-off between phage resistance and antibiotic resistance.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Significance
Bacteriophages (“phages,” viruses that infect bacteria) are an important source of selection for bacterial populations. Phages use various structures to infect bacterial cells, and bacteria often evolve phage resistance by losing or modifying these structures. We examine a phage that uses two structures that also provide
Escherichia coli
cells with antibiotic resistance. We show that phage selection can result in bacteria evolving phage resistance by losing or modifying the structures. When phage resistance evolves, the bacteria sometimes also show increased antibiotic sensitivity. This result indicates an evolutionary trade-off between phage resistance and antibiotic resistance. However, we also discovered bacterial mutations that avoid the trade-off. We discuss the potential use of phage selection and evolutionary trade-offs in treating bacterial infections.
|
| Volume |
117(21)
|
| Pages |
11207-11216
|
| Published |
2020-5-26
|
| DOI |
10.1073/pnas.1919888117
|
| PII |
1919888117
|
| PMID |
32424102
|
| PMC |
PMC7260982
|
| MeSH |
Anti-Bacterial Agents / pharmacology
Bacterial Outer Membrane Proteins / genetics
Coliphages / physiology*
Drug Resistance, Bacterial / physiology*
Escherichia coli / drug effects*
Escherichia coli / physiology*
Escherichia coli / virology
Escherichia coli Proteins / genetics
Gene Library
Genes, Bacterial
Genetic Pleiotropy*
Host Specificity
Lipopolysaccharides / genetics
Lipopolysaccharides / metabolism
Membrane Transport Proteins / genetics
Mutation
|
| IF |
9.412
|
| Resource |
| Prokaryotes E. coli |
JW5503-KC
JW2341-KC
JW0401-KC
JW3996-KC
JW0940-KC
JW2203-KC
JW5503-AM
JW3596-AM
JW3024-AM
JW3605-AM |
