RRC ID 70681
Author Wang T, Zhou M, Zhang Q, Zhang C, Peng G.
Title ubtor Mutation Causes Motor Hyperactivity by Activating mTOR Signaling in Zebrafish.
Journal Neurosci Bull
Abstract Mechanistic target of rapamycin (mTOR) signaling governs important physiological and pathological processes key to cellular life. Loss of mTOR negative regulators and subsequent over-activation of mTOR signaling are major causes underlying epileptic encephalopathy. Our previous studies showed that UBTOR/KIAA1024/MINAR1 acts as a negative regulator of mTOR signaling, but whether UBTOR plays a role in neurological diseases remains largely unknown. We therefore examined a zebrafish model and found that ubtor disruption caused increased spontaneous embryonic movement and neuronal activity in spinal interneurons, as well as the expected hyperactivation of mTOR signaling in early zebrafish embryos. In addition, mutant ubtor larvae showed increased sensitivity to the convulsant pentylenetetrazol, and both the motor activity and the neuronal activity were up-regulated. These phenotypic abnormalities in zebrafish embryos and larvae were rescued by treatment with the mTORC1 inhibitor rapamycin. Taken together, our findings show that ubtor regulates motor hyperactivity and epilepsy-like behaviors by elevating neuronal activity and activating mTOR signaling.
Volume 37(12)
Pages 1658-1670
Published 2021-12-1
DOI 10.1007/s12264-021-00755-z
PII 10.1007/s12264-021-00755-z
PMID 34309811
PMC PMC8643380
MeSH Animals Hyperkinesis* / genetics Mutation / genetics Signal Transduction TOR Serine-Threonine Kinases / genetics TOR Serine-Threonine Kinases / metabolism Zebrafish* / metabolism
IF 4.326
Zebrafish Tg(UAS:EGFP)