RRC ID 70780
Author Matsuda S, Schaefer JV, Mii Y, Hori Y, Bieli D, Taira M, Plückthun A, Affolter M.
Title Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc.
Journal Nat Commun
Abstract How morphogen gradients control patterning and growth in developing tissues remains largely unknown due to lack of tools manipulating morphogen gradients. Here, we generate two membrane-tethered protein binders that manipulate different aspects of Decapentaplegic (Dpp), a morphogen required for overall patterning and growth of the Drosophila wing. One is "HA trap" based on a single-chain variable fragment (scFv) against the HA tag that traps HA-Dpp to mainly block its dispersal, the other is "Dpp trap" based on a Designed Ankyrin Repeat Protein (DARPin) against Dpp that traps Dpp to block both its dispersal and signaling. Using these tools, we found that, while posterior patterning and growth require Dpp dispersal, anterior patterning and growth largely proceed without Dpp dispersal. We show that dpp transcriptional refinement from an initially uniform to a localized expression and persistent signaling in transient dpp source cells render the anterior compartment robust against the absence of Dpp dispersal. Furthermore, despite a critical requirement of dpp for the overall wing growth, neither Dpp dispersal nor direct signaling is critical for lateral wing growth after wing pouch specification. These results challenge the long-standing dogma that Dpp dispersal is strictly required to control and coordinate overall wing patterning and growth.
Volume 12(1)
Pages 6435
Published 2021-11-8
DOI 10.1038/s41467-021-26726-6
PII 10.1038/s41467-021-26726-6
PMID 34750371
PMC PMC8576045
MeSH Animals Animals, Genetically Modified Body Patterning / genetics Bone Morphogenetic Proteins / genetics Bone Morphogenetic Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Imaginal Discs / growth & development Imaginal Discs / metabolism Microscopy, Confocal Mutation Signal Transduction / genetics Wings, Animal / growth & development Wings, Animal / metabolism*
IF 12.121
Drosophila DGRC#101157