論文 - 詳細
| RRC ID | 70780 |
|---|---|
| 著者 | Matsuda S, Schaefer JV, Mii Y, Hori Y, Bieli D, Taira M, Plückthun A, Affolter M. |
| タイトル | Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc. |
| ジャーナル | Nat Commun |
| Abstract |
How morphogen gradients control patterning and growth in developing tissues remains largely unknown due to lack of tools manipulating morphogen gradients. Here, we generate two membrane-tethered protein binders that manipulate different aspects of Decapentaplegic (Dpp), a morphogen required for overall patterning and growth of the Drosophila wing. One is "HA trap" based on a single-chain variable fragment (scFv) against the HA tag that traps HA-Dpp to mainly block its dispersal, the other is "Dpp trap" based on a Designed Ankyrin Repeat Protein (DARPin) against Dpp that traps Dpp to block both its dispersal and signaling. Using these tools, we found that, while posterior patterning and growth require Dpp dispersal, anterior patterning and growth largely proceed without Dpp dispersal. We show that dpp transcriptional refinement from an initially uniform to a localized expression and persistent signaling in transient dpp source cells render the anterior compartment robust against the absence of Dpp dispersal. Furthermore, despite a critical requirement of dpp for the overall wing growth, neither Dpp dispersal nor direct signaling is critical for lateral wing growth after wing pouch specification. These results challenge the long-standing dogma that Dpp dispersal is strictly required to control and coordinate overall wing patterning and growth. |
| 巻・号 | 12(1) |
| ページ | 6435 |
| 公開日 | 2021-11-8 |
| DOI | 10.1038/s41467-021-26726-6 |
| PII | 10.1038/s41467-021-26726-6 |
| PMID | 34750371 |
| PMC | PMC8576045 |
| MeSH | Animals Animals, Genetically Modified Body Patterning / genetics Bone Morphogenetic Proteins / genetics Bone Morphogenetic Proteins / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental Imaginal Discs / growth & development Imaginal Discs / metabolism Microscopy, Confocal Mutation Signal Transduction / genetics Wings, Animal / growth & development Wings, Animal / metabolism* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#101157 |