RRC ID 70844
Author Nguyen TTN, Shim J, Song YH.
Title Chk2-p53 and JNK in irradiation-induced cell death of hematopoietic progenitors and differentiated cells in Drosophila larval lymph gland.
Journal Biol Open
Abstract Ionizing radiation (IR) induces DNA double-strand breaks that activate the DNA damage response (DDR), which leads to cell cycle arrest, senescence, or apoptotic cell death. Understanding the DDR of stem cells is critical to tissue homeostasis and the survival of the organism. Drosophila hematopoiesis serves as a model system for sensing stress and environmental changes; however, their response to DNA damage remains largely unexplored. The Drosophila lymph gland is the larval hematopoietic organ, where stem-like progenitors proliferate and differentiate into mature blood cells called hemocytes. We found that apoptotic cell death was induced in progenitors and hemocytes after 40 Gy irradiation, with progenitors showing more resistance to IR-induced cell death compared to hemocytes at a lower dose. Furthermore, we found that Drosophila ATM (tefu), Chk2 (lok), p53, and reaper were necessary for IR-induced cell death in the progenitors. Notably, IR-induced cell death in mature hemocytes required tefu, Drosophila JNK (bsk), and reaper, but not lok or p53. In summary, we found that DNA damage induces apoptotic cell death in the late third instar larval lymph gland and identified lok/p53-dependent and -independent cell death pathways in progenitors and mature hemocytes, respectively.
Volume 10(8)
Published 2021-8-15
DOI 10.1242/bio.058809
PII 271116
PMID 34328173
PMC PMC8411456
MeSH Animals Biomarkers Cell Differentiation* / radiation effects Checkpoint Kinase 2 / genetics* Checkpoint Kinase 2 / metabolism DNA Damage Drosophila / physiology* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / metabolism* Hemocytes JNK Mitogen-Activated Protein Kinases / genetics* JNK Mitogen-Activated Protein Kinases / metabolism Larva Radiation, Ionizing Tumor Suppressor Protein p53 / genetics*
IF 2.029
Drosophila DGRC#204829