RRC ID 7087
著者 Marteinsson VT, Bjornsdottir SH, Bienvenu N, Kristjansson JK, Birrien JL.
タイトル Rhodothermus profundi sp. nov., a thermophilic bacterium isolated from a deep-sea hydrothermal vent in the Pacific Ocean.
ジャーナル Int J Syst Evol Microbiol
Abstract Nine thermophilic strains of aerobic, non-sporulating, heterotrophic bacteria were isolated after enrichment of chimney material sampled from a deep-sea hydrothermal field at a depth of 2634m on the East-Pacific Rise (1 °N). The bacteria stained Gram-negative. They were rod-shaped and measured approximately 0.5μm in width and 1.5-3.5μm in length. They grew at 55-80°C, pH 6-8 and 1-6 % NaCl. Optimal growth was observed at 70-75°C, pH7.0 and 1-3 % NaCl. The organisms were identified as members of the genus Rhodothermus, having a 16S rRNA gene similarity of 98.1 % with Rhodothermus marinus DSM 4252(T). The novel isolates differed morphologically, physiologically and chemotaxonomically from R. marinus, e.g. in lack of pigmentation, response to hydrostatic pressure, maximum growth temperature and DNA G+C content. DNA-DNA hybridization revealed a reassociation value of 37.2 % between strain PRI 2902(T) and R. marinus DSM 4252(T), which strongly suggested that they represent different species. Furthermore, AFLP fingerprinting separated the novel strains from R. marinus reference strains. It is therefore concluded that the strains described here should be classified as representatives of a novel species for which the name Rhodothermus profundi sp. nov. is proposed; the type strain is PRI 2902(T) (=DSM 22212(T) =JCM 15944(T)).
巻・号 60(Pt 12)
ページ 2729-2734
公開日 2010-12-1
DOI 10.1099/ijs.0.012724-0
PMID 20061490
MeSH Amplified Fragment Length Polymorphism Analysis Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Hydrothermal Vents / microbiology* Molecular Sequence Data Nucleic Acid Amplification Techniques Pacific Ocean Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Rhodothermus / classification* Rhodothermus / genetics Rhodothermus / isolation & purification Seawater / microbiology* Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 20
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 15944