| RRC ID |
71109
|
| Author |
Seo B, Jeon K, Baek I, Lee YM, Baek K, Ko G.
|
| Title |
Clostridium fessum sp. nov., isolated from human faeces.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
An obligately anaerobic, Gram-stain-positive and spore-forming strain, SNUG30386T was isolated from a faecal sample of a healthy Korean subject. The strain formed a round ivory-coloured colony and cells were chained rods with tapered ends, approximately 2.0-2.5×0.6-0.8 μm in size. The taxonomic analysis indicated that strain SNUG30386T was within the family Lachnospiraceae. According to the 16S rRNA gene sequence similarity, the closest species to strain SNUG30386T was Clostridium symbiosum (95.6 %), followed by Enterocloster asparagiformis (94.8 %), Enterocloster clostridioformis (94.8 %) and Enterocloster lavalensis (94.6 %). The evolutionary tree based on 16S rRNA gene sequences demonstrated that strain SNUG30386T had split apart at a unique branch point far from other close relatives. Its DNA G+C content was 48.3 mol% calculated from the whole genome sequence. The major cellular fatty acids were C16 : 0 and C14 : 0. Compared to those of the closely related species, strain SNUG30386T showed distinct biochemical activities such as being unable to utilize most of carbon sources except d-glucose and l-arabinose. As a result, based on its unique phylogenetic clade and taxonomic characteristics, we conclude that strain SNUG30386T represents a novel species within the genus Clostridium, for which the name Clostridium fessum sp. nov. is proposed. The type strain of the novel species is SNUG30386T (=KCTC 15633T= JCM 32258T).
|
| Volume |
71(1)
|
| Published |
2021-1-1
|
| DOI |
10.1099/ijsem.0.004579
|
| PMID |
33253079
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Clostridium / classification*
Clostridium / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Humans
Phylogeny*
RNA, Ribosomal, 16S / genetics
Republic of Korea
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM32258 |
