| 著者 |
Zhang G, Yang R, Chen T, Zhang B, Yang H, Wu X, Gao H, Zhang W, Liu G.
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| Abstract |
A bacterial strain, designated Y40T, was isolated from sandy soil sampled on the Qinghai-Tibet Plateau. A polyphasic study confirmed the affiliation of the strain with the genus Mesobacillus. Strain Y40T was found to be an aerobic, Gram-stain-positive, motile and rod-shaped bacterium. The strain grew at 10-42 °C, pH 6-9 and with 0-2 % (w/v) NaCl. The diagnostic amino acid was meso-diaminopimeilic acid. MK7 was predominant menaquinone, and iso-C15:0, iso-C17:1 ω10c and anteiso-C15:0 were the major fatty acids. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The DNA G+C content was 40.6 mol%. Based on he results of 16S rRNA gene sequence analysis, strain Y40T was phylogenetically closely related to Mesobacillus zeae JJ-247T and Mesobacillus foraminis CV53T, with similarities of 98.0 and 97.7 %, respectively. The average nucleotide identity (ANIb) values between strain Y40T and Mesobacillus zeae JJ-247T and Mesobacillus foraminis CV53T were 69.9 and 70.0 %, respectively. Based on the morphological, physiological, and chemotaxonomic data, it is proposed that strain Y40T (=CICC 24459T=JCM 32794T) should be classified into the genus Mesobacillus as Mesobacillus harenae sp. nov.
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