| RRC ID |
71179
|
| Author |
Pitt A, Schmidt J, Koll U, Hahn MW.
|
| Title |
Aquiluna borgnonia gen. nov., sp. nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria. The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna. Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1. The genome-based phylogenetic tree showed that the new strain represents a putative new genus. Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells. They metabolized a broad variety of substrates. Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0. The major respiratory quinone was MK-11 and a minor component was MK-10. The peptidoglycan structure belonged to an unusual B type. The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%. An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome. Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
|
| Volume |
71(5)
|
| Published |
2021-5-1
|
| DOI |
10.1099/ijsem.0.004825
|
| PMID |
33999796
|
| MeSH |
Actinobacteria / classification*
Actinobacteria / isolation & purification
Austria
Bacterial Typing Techniques
Base Composition
Cell Wall / chemistry
DNA, Bacterial / genetics
Fatty Acids / chemistry
Fresh Water / microbiology*
Genome Size
Peptidoglycan / chemistry
Phylogeny*
Pigmentation
Ponds / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Vitamin K 2 / chemistry
|
| Resource |
| General Microbes |
JCM32974 |
