論文 - 詳細
| RRC ID | 71181 |
|---|---|
| 著者 | Ming H, Cheng LJ, Yi BF, Xia TT, Niu MM, Zhao ZY, Liu BB, Nie GX, Cui CX. |
| タイトル | Brachybacterium subflavum sp. nov., a novel actinobacterium isolated from the foregut of grass carp. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel actinobacterium, designated CFH 10395T, was isolated from the foregut of grass carp (Ctenopharyngodon idella), which had been fed with ginseng extract supplement. The taxonomic position was investigated by a polyphasic approach. Cells of CFH 10395T were Gram-staining-positive, aerobic, ovoid-shaped, non-spore-forming and non-motile. On the basis of the results of 16S rRNA gene sequence analysis, CFH 10395T was most closely related to Brachybacterium endophyticum KCTC 49087T, Brachybacterium squillarum JCM 16464T and Brachybacterium paraconglomeratum JCM 17781T (97.85%, 97.51 and 97.29% similarity, respectively). CFH 10395T grew at 4-37 °C, pH 5.0-9.0 and in the presence of up to 10.0 % NaCl (w/v). The dominant menaquinone was MK-7. The whole-cell sugars were rhamnose, glucose, mannose and galactose. meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genome size was 3.99 Mbp with a DNA G+C content of 71.9 mol%. On the basis of the results of phylogenetic analysis, physiological properties, chemotaxonomic characteristics, low average nucleotide identity (ANI) and digital DDH (dDDH) results [ANI calculated using MUMmer (ANIm) <87 %, ANI calculated using blast (ANIb) <83 % and dDDH <23 %], it is concluded that CFH 10395T represents a novel species of the genus Brachybacterium, for which the name Brachybacterium subflavum sp. nov., is proposed. The type strain is CFH 10395T (=CGMCC 1.13804T=KCTC 49235T). |
| 巻・号 | 71(6) |
| 公開日 | 2021-6-1 |
| DOI | 10.1099/ijsem.0.004839 |
| PMID | 34170217 |
| MeSH | Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Animals Bacterial Typing Techniques Base Composition Carps / microbiology* DNA, Bacterial / genetics Fatty Acids / analysis Fatty Acids / chemistry Nucleic Acid Hybridization Phospholipids / analysis Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM16464 JCM17781 |