| RRC ID |
71182
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| 著者 |
Ge Y, Yang J, Lai XH, Jin D, Lu S, Zhang G, Huang Y, Luo X, Zheng H, Tao Y, Yuan T, Li L, Zhang X, Xu J.
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| タイトル |
Enorma shizhengliae sp. nov. and Eggerthella guodeyinii sp. nov., two new members of the family Coriobacteriaceae.
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| ジャーナル |
Int J Syst Evol Microbiol
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| Abstract |
Four obligatory anaerobic, Gram-stain-positive, non-motile and rod-shaped organisms (HF-1365T, HF-1362, HF-1101T and HF-4214) were isolated from faecal samples of healthy Chinese subjects. Results of 16S rRNA gene sequence analyses showed that these isolates belong to the genera Enorma (strains HF-1365T and HF-1362) and Eggerthella (strains HF-1101T and HF-4214), closest to Enorma massiliensis (both 98.6 %) and Eggerthella sinensis (98.0 and 97.8 %), respectively. The whole genome sequences of strains HF-1365T and HF-1101T were 2.3 and 4.2 Mb in size with 61.7 and 66.2 mol% DNA G+C content, respectively. The average nucleotide identity and digital DNA-DNA hybridization values indicated that strains HF-1365T and HF-1101T represent novel species in the genera Enorma and Eggerthella. Major fatty acid constituents (>10 %) of strains HF-1365T and HF-1362 were C12 : 0 (24.7 and 23.9 %), C14 : 0 (21.9 and 20.6 %) and summed feature 1 (C15 : 1iso H/C13 : 0 3OH; 12.8 and 10.8 %); those of strains HF-1101T and HF-4214 were C18 : 1 ω9c (32.4 and 33.1 %) and C16 : 0 (13.9 and 14.0 %). Strain HF-1365T had phospholipid, glycolipid, lipid and phosphoglycolipid without any known quinones, while strain HF-1101T had diphosphatidylglycerol as the major polar lipid and MK-7 (80.7 %) as the predominant quinone. On the basis of their phylogenetic and phenotypic characteristics, strains HF-1365T and HF-1101T represent two distinct species, respectively, in the genera Enorma and Eggerthella, for which the names Enorma shizhengliae sp. nov. (type strain HF-1365T=CGMCC 1.17435T=GDMCC 1.1705T=JCM 33601T) and Eggerthella guodeyinii sp. nov. (type strain HF-1101T=CGMCC 1.17436T=GDMCC 1.1668T=JCM 33773T) are proposed.
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| 巻・号 |
71(7)
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| 公開日 |
2021-7-1
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| DOI |
10.1099/ijsem.0.004840
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| PMID |
34255621
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| MeSH |
Actinobacteria / classification*
Actinobacteria / isolation & purification
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Humans
Nucleic Acid Hybridization
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
| リソース情報 |
| 一般微生物 |
JCM33601
JCM33773 |
