RRC ID 71219
Author Kobayashi H, Tanizawa Y, Sakamoto M, Ohkuma M, Tohno M.
Title Taxonomic status of the species Clostridium methoxybenzovorans Mechichi et al. 1999.
Journal Int J Syst Evol Microbiol
Abstract The taxonomic status of the species Clostridium methoxybenzovorans was assessed. The 16S rRNA gene sequence, whole-genome sequence and phenotypic characterizations suggested that the type strain deposited in the American Type Culture Collection (C. methoxybenzovorans ATCC 700855T) is a member of the species Eubacterium callanderi. Hence, C. methoxybenzovorans ATCC 700855T cannot be used as a reference for taxonomic study. The type strain deposited in the German Collection of Microorganism and Cell Cultures GmbH (DSM 12182T) is no longer listed in its online catalogue. Also, both the 16S rRNA gene and the whole-genome sequences of the original strain SR3T showed high sequence identity with those of Lacrimispora indolis (recently reclassified from Clostridium indolis) as the most closely related species. Analysis of the two genomes showed average nucleotide identity based on blast and digital DNA-DNA hybridization values of 98.3 and 87.9 %, respectively. Based on these results, C. methoxybenzovorans SR3T was considered to be a member of L. indolis.
Volume 71(8)
Published 2021-8-1
DOI 10.1099/ijsem.0.004951
PMID 34379581
MeSH Bacterial Typing Techniques Base Composition Clostridium* / classification Clostridium* / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
Resource
General Microbes JCM10284