論文 - 詳細
| RRC ID | 71236 |
|---|---|
| 著者 | Phongsopitanun W, Kanchanasin P, Sripreechasak P, Rueangsawang K, Athipornchai A, Supong K, Pittayakhajonwut P, Tanasupawat S. |
| タイトル | Potential antibiotic production of Streptomyces justiciae sp. nov., isolated from the root of Justicia subcoriacea. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Endophytic actinobacterial strain 3R004T was isolated from a root of Justicia subcoriacea collected in Thailand. In this report, the taxonomic position of this strain is described using a polyphasic approach. Based on the morphological characteristics and chemical composition of its cells, strain 3R004T was identified as a member of the genus Streptomyces. It produced a long chain of cylindrical spores on aerial mycelia. ll-Diaminopimelic acid was detected in the cell wall peptidoglycan. The menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0 were detected as the major cellular fatty acids. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and one unidentified lipid. Strain 3R004T showed the highest 16S rRNA gene similarity of 99.45 % to Streptomyces cyaneochromogenes MK-45T. The phylogenomic results indicated that strain 3R004T was close to Streptomyces aquilus GGCR-6T and Streptomyces antibioticus DSM 40234T. The DNA-DNA hybridization and average nucleotide identity values among strain 3R004T and closely related Streptomyces species were 35.5-63.1 % and 82.7-94.3 %, respectively. The type strain produced actinomycin D antibiotic as the major secondary metabolite. The maximum productivity of the actinomycin D (378 mg l-1) was observed when the strain was grown in 301 broth at 30 °C, 180 r.p.m. for 12 days. On the basis of phenotypic and genotypic evidence, strain 3R004T represents a novel species of the genus Streptomyces, for which the name Streptomyces justiciae is proposed. The type strain is 3R004T (=LMG 32138T=TBRC 13128T=NBRC 115065T). |
| 巻・号 | 71(9) |
| 公開日 | 2021-9-1 |
| DOI | 10.1099/ijsem.0.005017 |
| PMID | 34550061 |
| MeSH | Anti-Bacterial Agents Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Justicia* Phospholipids Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Streptomyces* / genetics Vitamin K 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM4620 JCM33584 |