| RRC ID |
71255
|
| Author |
Zakharyuk A, Valyshev A, Plotnikov A, Kopitsyn D, Suzina N, Shcherbakova V.
|
| Title |
Trichococcus shcherbakoviae subsp. psychrophilus subsp. nov., a psychrotolerant facultative anaerobe isolated from a cold spring.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A psychrotolerant facultative anaerobe, strain SKBGT, was isolated from the bottom sediments of the cold mineral spring Buxichen (Buryatia, Russia). Gram-positive non-motile cocci with a diameter of 1.75-2.5 µm were observed singly or in long chains. Cells grew in the temperature range from ̶ 5-35 °C. Growth was observed within the pH range of 7.0-9.5, with the optimum growth at pH 7.6 and at a NaCl concentration from 0-1.0 % (optimum 0.1 % (w/v)). Strain SKBGT was a chemoorganoheterotroph that used sugars and some organic acids as substrates. The predominant fatty acids in cell walls were С16:1ω9, С18:1ω9, and С16 : 0. The 16S rRNA gene sequence of strain SKBGT shared high similarity (>99 %) with those of the type strains of the genus Trichococcus. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKBGT and Trichococcus shcherbakoviae ArtT (=DSM 107162T=VKM B-3260T) were 70.1 and 95.4 %, respectively. The genomic DNA G+C content of strain SKBGT was 47.1 mol%. Compared with the type strain of T. shcherbakoviae, the new strain was characterized by a temperature optimum for growth (10 °C) significantly lower than that of T. shcherbakoviae DSM 107162T (20-30 °C). Based on phenotypic and genomic characteristics, the isolate SKBGT was classified as T. shcherbakoviae subsp. psychrophilus subsp. nov. The type strain is SKBGT (=VKM B-3241Т=JCM 33326T).
|
| Volume |
71(10)
|
| Published |
2021-10-1
|
| DOI |
10.1099/ijsem.0.005068
|
| PMID |
34694985
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Carnobacteriaceae / classification*
Carnobacteriaceae / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Natural Springs / microbiology*
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Russia
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM33326 |
