論文 - 詳細
| RRC ID | 71259 |
|---|---|
| 著者 | Hashimoto Y, Tame A, Sawayama S, Miyazaki J, Takai K, Nakagawa S. |
| タイトル | Desulfomarina profundi gen. nov., sp. nov., a novel mesophilic, hydrogen-oxidizing, sulphate-reducing chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel mesophilic, strictly anaerobic, chemolithoautotrophic sulphate-reducing bacterium, designated strain KT2T, was isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc. Strain KT2T grew at 25-40 °C (optimum 35 °C) and pH 5.5-7.0 (optimum 6.6) in the presence of 25-45 g l-1 NaCl (optimum 30 g l-1). Growth occurred with molecular hydrogen as the electron donor and sulphate, thiosulphate, and sulphite as the electron acceptors. The isolate utilized CO2 as the sole carbon source for chemolithoautotrophic growth on H2. Glycerol, succinate, fumarate, malate, glutamate, or casamino acids could serve as an alternative electron donor in the presence of CO2. Malate, citrate, glutamate, and casamino acids were used as fermentative substrates for weak growth. The G+C content of genomic DNA was 46.1 %. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain KT2T is a member of the family Desulfobulbaceae, showing a sequence similarity of 94.3 % with Desulforhopalus singaporensis. Phylogenomic analysis based on concatenated 156 single-copy marker genes confirmed the same topology as the 16S rRNA gene phylogeny. The ANI and AAI values between strain KT2T and related genera of the family Desulfobulbaceae were 65.6-68.6 % and 53.1-62.9 %. Based on the genomic, molecular, and physiological characteristics, strain KT2T represents a novel genus and species within the family Desulfobulbaceae, for which the name Desulfomarina profundi gen. nov., sp. nov. is proposed, with KT2T (=JCM 34118T = DSM 111364T) as the type strain. |
| 巻・号 | 71(11) |
| 公開日 | 2021-11-1 |
| DOI | 10.1099/ijsem.0.005083 |
| PMID | 34739365 |
| MeSH | Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Deltaproteobacteria / classification* Deltaproteobacteria / isolation & purification Fatty Acids / chemistry Hydrogen Hydrothermal Vents* / microbiology Oxidation-Reduction Phylogeny* RNA, Ribosomal, 16S / genetics Seawater / microbiology Sequence Analysis, DNA Sulfates Sulfur-Reducing Bacteria / classification Sulfur-Reducing Bacteria / isolation & purification |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 41 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM34118 JCM14043 |